(Per)chlorate reduction by an acetogenic bacterium, Sporomusa sp., isolated from an underground gas storage

M. Balk, F. Mehboob, T. Van Gelder, W.I.C. Rijpstra, J.S. Sinninghe Damsté, A.J.M. Stams

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    A mesophilic bacterium, strain An4, was isolated from an underground gas storage reservoir with methanol as substrate and perchlorate as electron acceptor. Cells were Gram-negative, spore-forming, straight to curved rods, 0.5–0.8 μm in diameter, and 2–8 μm in length, growing as single cells or in pairs. The cells grew optimally at 37°C, and the pH optimum was around 7. Strain An4 converted various alcohols, organic acids, fructose, acetoin, and H2/CO2 to acetate, usually as the only product. Succinate was decarboxylated to propionate. The isolate was able to respire with (per)chlorate, nitrate, and CO2. The G+C content of the DNA was 42.6 mol%. Based on the 16S rRNA gene sequence analysis, strain An4 was most closely related to Sporomusa ovata (98% similarity). The bacterium reduced perchlorate and chlorate completely to chloride. Key enzymes, perchlorate reductase and chlorite dismutase, were detected in cell-free extracts.
    Originele taal-2Engels
    Pagina's (van-tot)595-603
    TijdschriftApplied Microbiology and Biotechnology
    Volume88
    Nummer van het tijdschrift2
    DOI's
    StatusGepubliceerd - 2010

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    Balk, M., Mehboob, F., Van Gelder, T., Rijpstra, W. I. C., Sinninghe Damsté, J. S., & Stams, A. J. M. (2010). (Per)chlorate reduction by an acetogenic bacterium, Sporomusa sp., isolated from an underground gas storage. Applied Microbiology and Biotechnology, 88(2), 595-603. https://doi.org/10.1007/s00253-010-2788-8