(Per)chlorate reduction by an acetogenic bacterium, Sporomusa sp., isolated from an underground gas storage

M. Balk; F. Mehboob; T. Van Gelder; W.I.C. Rijpstra; J.S. Sinninghe Damsté; A.J.M. Stams

doi:10.1007/s00253-010-2788-8

(Per)chlorate reduction by an acetogenic bacterium, Sporomusa sp., isolated from an underground gas storage

M. Balk, F. Mehboob, T. Van Gelder, W.I.C. Rijpstra, J.S. Sinninghe Damsté, A.J.M. Stams

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

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A mesophilic bacterium, strain An4, was isolated from an underground gas storage reservoir with methanol as substrate and perchlorate as electron acceptor. Cells were Gram-negative, spore-forming, straight to curved rods, 0.5–0.8 μm in diameter, and 2–8 μm in length, growing as single cells or in pairs. The cells grew optimally at 37°C, and the pH optimum was around 7. Strain An4 converted various alcohols, organic acids, fructose, acetoin, and H2/CO2 to acetate, usually as the only product. Succinate was decarboxylated to propionate. The isolate was able to respire with (per)chlorate, nitrate, and CO2. The G+C content of the DNA was 42.6 mol%. Based on the 16S rRNA gene sequence analysis, strain An4 was most closely related to Sporomusa ovata (98% similarity). The bacterium reduced perchlorate and chlorate completely to chloride. Key enzymes, perchlorate reductase and chlorite dismutase, were detected in cell-free extracts.

Originele taal-2	Engels
Pagina's (van-tot)	595-603
Tijdschrift	Applied Microbiology and Biotechnology
Volume	88
Nummer van het tijdschrift	2
DOI's	https://doi.org/10.1007/s00253-010-2788-8
Status	Gepubliceerd - 2010

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10.1007/s00253-010-2788-8

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title = "(Per)chlorate reduction by an acetogenic bacterium, Sporomusa sp., isolated from an underground gas storage",

abstract = "A mesophilic bacterium, strain An4, was isolated from an underground gas storage reservoir with methanol as substrate and perchlorate as electron acceptor. Cells were Gram-negative, spore-forming, straight to curved rods, 0.5–0.8 μm in diameter, and 2–8 μm in length, growing as single cells or in pairs. The cells grew optimally at 37°C, and the pH optimum was around 7. Strain An4 converted various alcohols, organic acids, fructose, acetoin, and H2/CO2 to acetate, usually as the only product. Succinate was decarboxylated to propionate. The isolate was able to respire with (per)chlorate, nitrate, and CO2. The G+C content of the DNA was 42.6 mol%. Based on the 16S rRNA gene sequence analysis, strain An4 was most closely related to Sporomusa ovata (98% similarity). The bacterium reduced perchlorate and chlorate completely to chloride. Key enzymes, perchlorate reductase and chlorite dismutase, were detected in cell-free extracts.",

author = "M. Balk and F. Mehboob and {Van Gelder}, T. and W.I.C. Rijpstra and {Sinninghe Damst{\'e}}, J.S. and A.J.M. Stams",

note = "Reporting year: 2010 Metis note: 4770;CTE; ME; file:///L:\EndnoteDatabases\NIOOPUB\pdfs\PDFS2010\Balk_ea_4770.pdf",

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(Per)chlorate reduction by an acetogenic bacterium, Sporomusa sp., isolated from an underground gas storage. / Balk, M.; Mehboob, F.; Van Gelder, T.; Rijpstra, W.I.C.; Sinninghe Damsté, J.S.; Stams, A.J.M.

In: Applied Microbiology and Biotechnology, Vol. 88, Nr. 2, 2010, blz. 595-603.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

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AU - Balk, M.

AU - Mehboob, F.

AU - Van Gelder, T.

AU - Rijpstra, W.I.C.

AU - Sinninghe Damsté, J.S.

AU - Stams, A.J.M.

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N2 - A mesophilic bacterium, strain An4, was isolated from an underground gas storage reservoir with methanol as substrate and perchlorate as electron acceptor. Cells were Gram-negative, spore-forming, straight to curved rods, 0.5–0.8 μm in diameter, and 2–8 μm in length, growing as single cells or in pairs. The cells grew optimally at 37°C, and the pH optimum was around 7. Strain An4 converted various alcohols, organic acids, fructose, acetoin, and H2/CO2 to acetate, usually as the only product. Succinate was decarboxylated to propionate. The isolate was able to respire with (per)chlorate, nitrate, and CO2. The G+C content of the DNA was 42.6 mol%. Based on the 16S rRNA gene sequence analysis, strain An4 was most closely related to Sporomusa ovata (98% similarity). The bacterium reduced perchlorate and chlorate completely to chloride. Key enzymes, perchlorate reductase and chlorite dismutase, were detected in cell-free extracts.

AB - A mesophilic bacterium, strain An4, was isolated from an underground gas storage reservoir with methanol as substrate and perchlorate as electron acceptor. Cells were Gram-negative, spore-forming, straight to curved rods, 0.5–0.8 μm in diameter, and 2–8 μm in length, growing as single cells or in pairs. The cells grew optimally at 37°C, and the pH optimum was around 7. Strain An4 converted various alcohols, organic acids, fructose, acetoin, and H2/CO2 to acetate, usually as the only product. Succinate was decarboxylated to propionate. The isolate was able to respire with (per)chlorate, nitrate, and CO2. The G+C content of the DNA was 42.6 mol%. Based on the 16S rRNA gene sequence analysis, strain An4 was most closely related to Sporomusa ovata (98% similarity). The bacterium reduced perchlorate and chlorate completely to chloride. Key enzymes, perchlorate reductase and chlorite dismutase, were detected in cell-free extracts.

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DO - 10.1007/s00253-010-2788-8

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