Phylogenetic diversity of human pathogenic Fusarium and emergence of uncommon virulent species

Husam Salah; Abdullah M S Al-Hatmi; Bart Theelen; Mohammed Abukamar; Samar Hashim; Anne D van Diepeningen; Cornelia Lass-Florl; Teun Boekhout; Muna Almaslamani; Saad J Taj-Aldeen

doi:10.1016/j.jinf.2015.08.011

Phylogenetic diversity of human pathogenic Fusarium and emergence of uncommon virulent species

Husam Salah, Abdullah M S Al-Hatmi, Bart Theelen, Mohammed Abukamar, Samar Hashim, Anne D van Diepeningen, Cornelia Lass-Florl, Teun Boekhout, Muna Almaslamani, Saad J Taj-Aldeen

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

43 Citaten (Scopus)

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OBJECTIVES: Fusarium species cause a broad spectrum of infections. However, little is known about the etiological agents to the species level. We identified Fusarium species isolated from clinical specimens including those of high risk patients to better understand the species involved in the pathogenesis.

METHODS: A set of 44 Fusarium isolates were identified by two-locus sequence typing using partial sequences of the second largest subunit of RNA polymerase (RPB2) and translation elongation factor 1 alpha (TEF-1α).

RESULTS: The identified species belonged to four species complexes (SC); the most common SC was Fusarium solani (FSSC) (75%), followed by Fusarium oxysporum (FOSC) (4.5%), Fusarium fujikuroi (FFSC) (13.6%), and Fusarium dimerum (FDSC) (6.8%). Sites of infections were nails (n = 19, 43.2%), skin (n = 7, 15.9%), cornea (n = 6, 13.6%), blood (n = 3, 9%), wound (n = 4, 6.8%), burn (n = 2, 4.5%), tissue (n = 2, 4.5%), and urine (n = 1, 2.27%). Fusarium acutatum was rare and seem restricted to the Middle East. Comorbidities associated with invasive infections were hematological malignancy and autoimmune disorders.

CONCLUSIONS: Members of the FSSC predominantly caused cornea, nail and bloodstream infections. Less frequently encountered were the FOSC, FFSC and FDSC. More accurate molecular identification of Fusarium species is important to predict therapeutic outcome and the emergence of these species.

Originele taal-2	Engels
Pagina's (van-tot)	658-66
Aantal pagina's	9
Tijdschrift	The Journal of infection
Volume	71
Nummer van het tijdschrift	6
DOI's	https://doi.org/10.1016/j.jinf.2015.08.011
Status	Gepubliceerd - dec. 2015

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10.1016/j.jinf.2015.08.011

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@article{dd522c51a8dc4ec79465222ec9507c7b,

title = "Phylogenetic diversity of human pathogenic Fusarium and emergence of uncommon virulent species",

abstract = "OBJECTIVES: Fusarium species cause a broad spectrum of infections. However, little is known about the etiological agents to the species level. We identified Fusarium species isolated from clinical specimens including those of high risk patients to better understand the species involved in the pathogenesis.METHODS: A set of 44 Fusarium isolates were identified by two-locus sequence typing using partial sequences of the second largest subunit of RNA polymerase (RPB2) and translation elongation factor 1 alpha (TEF-1α).RESULTS: The identified species belonged to four species complexes (SC); the most common SC was Fusarium solani (FSSC) (75%), followed by Fusarium oxysporum (FOSC) (4.5%), Fusarium fujikuroi (FFSC) (13.6%), and Fusarium dimerum (FDSC) (6.8%). Sites of infections were nails (n = 19, 43.2%), skin (n = 7, 15.9%), cornea (n = 6, 13.6%), blood (n = 3, 9%), wound (n = 4, 6.8%), burn (n = 2, 4.5%), tissue (n = 2, 4.5%), and urine (n = 1, 2.27%). Fusarium acutatum was rare and seem restricted to the Middle East. Comorbidities associated with invasive infections were hematological malignancy and autoimmune disorders.CONCLUSIONS: Members of the FSSC predominantly caused cornea, nail and bloodstream infections. Less frequently encountered were the FOSC, FFSC and FDSC. More accurate molecular identification of Fusarium species is important to predict therapeutic outcome and the emergence of these species.",

author = "Husam Salah and Al-Hatmi, {Abdullah M S} and Bart Theelen and Mohammed Abukamar and Samar Hashim and {van Diepeningen}, {Anne D} and Cornelia Lass-Florl and Teun Boekhout and Muna Almaslamani and Taj-Aldeen, {Saad J}",

year = "2015",

month = dec,

doi = "10.1016/j.jinf.2015.08.011",

language = "English",

volume = "71",

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TY - JOUR

T1 - Phylogenetic diversity of human pathogenic Fusarium and emergence of uncommon virulent species

AU - Salah, Husam

AU - Al-Hatmi, Abdullah M S

AU - Theelen, Bart

AU - Abukamar, Mohammed

AU - Hashim, Samar

AU - van Diepeningen, Anne D

AU - Lass-Florl, Cornelia

AU - Boekhout, Teun

AU - Almaslamani, Muna

AU - Taj-Aldeen, Saad J

PY - 2015/12

Y1 - 2015/12

N2 - OBJECTIVES: Fusarium species cause a broad spectrum of infections. However, little is known about the etiological agents to the species level. We identified Fusarium species isolated from clinical specimens including those of high risk patients to better understand the species involved in the pathogenesis.METHODS: A set of 44 Fusarium isolates were identified by two-locus sequence typing using partial sequences of the second largest subunit of RNA polymerase (RPB2) and translation elongation factor 1 alpha (TEF-1α).RESULTS: The identified species belonged to four species complexes (SC); the most common SC was Fusarium solani (FSSC) (75%), followed by Fusarium oxysporum (FOSC) (4.5%), Fusarium fujikuroi (FFSC) (13.6%), and Fusarium dimerum (FDSC) (6.8%). Sites of infections were nails (n = 19, 43.2%), skin (n = 7, 15.9%), cornea (n = 6, 13.6%), blood (n = 3, 9%), wound (n = 4, 6.8%), burn (n = 2, 4.5%), tissue (n = 2, 4.5%), and urine (n = 1, 2.27%). Fusarium acutatum was rare and seem restricted to the Middle East. Comorbidities associated with invasive infections were hematological malignancy and autoimmune disorders.CONCLUSIONS: Members of the FSSC predominantly caused cornea, nail and bloodstream infections. Less frequently encountered were the FOSC, FFSC and FDSC. More accurate molecular identification of Fusarium species is important to predict therapeutic outcome and the emergence of these species.

AB - OBJECTIVES: Fusarium species cause a broad spectrum of infections. However, little is known about the etiological agents to the species level. We identified Fusarium species isolated from clinical specimens including those of high risk patients to better understand the species involved in the pathogenesis.METHODS: A set of 44 Fusarium isolates were identified by two-locus sequence typing using partial sequences of the second largest subunit of RNA polymerase (RPB2) and translation elongation factor 1 alpha (TEF-1α).RESULTS: The identified species belonged to four species complexes (SC); the most common SC was Fusarium solani (FSSC) (75%), followed by Fusarium oxysporum (FOSC) (4.5%), Fusarium fujikuroi (FFSC) (13.6%), and Fusarium dimerum (FDSC) (6.8%). Sites of infections were nails (n = 19, 43.2%), skin (n = 7, 15.9%), cornea (n = 6, 13.6%), blood (n = 3, 9%), wound (n = 4, 6.8%), burn (n = 2, 4.5%), tissue (n = 2, 4.5%), and urine (n = 1, 2.27%). Fusarium acutatum was rare and seem restricted to the Middle East. Comorbidities associated with invasive infections were hematological malignancy and autoimmune disorders.CONCLUSIONS: Members of the FSSC predominantly caused cornea, nail and bloodstream infections. Less frequently encountered were the FOSC, FFSC and FDSC. More accurate molecular identification of Fusarium species is important to predict therapeutic outcome and the emergence of these species.

U2 - 10.1016/j.jinf.2015.08.011

DO - 10.1016/j.jinf.2015.08.011

M3 - Article

C2 - 26348828

SN - 0163-4453

VL - 71

SP - 658

EP - 666

JO - The Journal of infection

JF - The Journal of infection

IS - 6

ER -

Phylogenetic diversity of human pathogenic Fusarium and emergence of uncommon virulent species

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