Samenvatting
Sequential 3'-to-5' activation of the Hox gene clusters in early embryos is a most fascinating issue in developmental biology. Neither the trigger nor the regulatory elements involved in the transcriptional initiation of the 3'-most Hox genes have been unraveled in any organism. We demonstrate that a series of enhancers, some of which are Wnt-dependent, is located within a HoxA 3' subtopologically associated domain (subTAD). This subTAD forms the structural basis for multiple layers of 3'-polarized features, including DNA accessibility and enhancer activation. Deletion of the cassette of Wnt-dependent enhancers proves its crucial role in initial transcription of HoxA at the 3' side of the cluster.
Originele taal-2 | Engels |
---|---|
Pagina's (van-tot) | 1937-42 |
Aantal pagina's | 6 |
Tijdschrift | Genes & Development |
Volume | 30 |
Nummer van het tijdschrift | 17 |
DOI's | |
Status | Gepubliceerd - 01 sep. 2016 |