Population size mediates the contribution of high-rate and large-benefit mutations to parallel evolution

Martijn F. Schenk; Mark Zwart; Sungmin Hwang; Philip Ruelens; Edouard Severing; Joachim Krug; J. Arjan G. M. de Visser

doi:10.1101/2021.02.02.429281

Population size mediates the contribution of high-rate and large-benefit mutations to parallel evolution

Martijn F. Schenk, Mark Zwart, Sungmin Hwang, Philip Ruelens, Edouard Severing, Joachim Krug, J. Arjan G. M. de Visser

NIOO - Microbial Ecology (ME)

Onderzoeksoutput: Working paper/discussiepaper › Voordruk › Wetenschappelijk

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Both mutations with large benefits and mutations occurring at high rates may cause parallel evolution, but their contribution is expected to depend on population size. We show that small and large bacterial populations adapt to a novel antibiotic using similar numbers, but different types of mutations. Small populations repeatedly substitute similar high-rate structural variants, including the deletion of a nonfunctional β-lactamase, and evolve modest resistance levels. Hundred-fold larger populations more frequently use the same low-rate, large-benefit point mutations, including those activating the β-lactamase, and reach 50-fold higher resistance levels. Our results demonstrate a key role of clonal interference in mediating the contribution of high-rate and large-benefit mutations in populations of different size, facilitated by a tradeoff between rates and fitness effects of different mutation classes.

Originele taal-2	Engels
Uitgever	bioRxiv
DOI's	https://doi.org/10.1101/2021.02.02.429281
Status	Gepubliceerd - 02 feb. 2021

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Naam	bioRxiv
Uitgeverij	Cold Spring Harbor Laboratory Press

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10.1101/2021.02.02.429281Licentie: CC BY

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abstract = "Both mutations with large benefits and mutations occurring at high rates may cause parallel evolution, but their contribution is expected to depend on population size. We show that small and large bacterial populations adapt to a novel antibiotic using similar numbers, but different types of mutations. Small populations repeatedly substitute similar high-rate structural variants, including the deletion of a nonfunctional β-lactamase, and evolve modest resistance levels. Hundred-fold larger populations more frequently use the same low-rate, large-benefit point mutations, including those activating the β-lactamase, and reach 50-fold higher resistance levels. Our results demonstrate a key role of clonal interference in mediating the contribution of high-rate and large-benefit mutations in populations of different size, facilitated by a tradeoff between rates and fitness effects of different mutation classes.",

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AU - Schenk, Martijn F.

AU - Zwart, Mark

AU - Hwang, Sungmin

AU - Ruelens, Philip

AU - Severing, Edouard

AU - Krug, Joachim

AU - de Visser, J. Arjan G. M.

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AB - Both mutations with large benefits and mutations occurring at high rates may cause parallel evolution, but their contribution is expected to depend on population size. We show that small and large bacterial populations adapt to a novel antibiotic using similar numbers, but different types of mutations. Small populations repeatedly substitute similar high-rate structural variants, including the deletion of a nonfunctional β-lactamase, and evolve modest resistance levels. Hundred-fold larger populations more frequently use the same low-rate, large-benefit point mutations, including those activating the β-lactamase, and reach 50-fold higher resistance levels. Our results demonstrate a key role of clonal interference in mediating the contribution of high-rate and large-benefit mutations in populations of different size, facilitated by a tradeoff between rates and fitness effects of different mutation classes.

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Population size mediates the contribution of high-rate and large-benefit mutations to parallel evolution

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