Potential for biocontrol of hairy root disease by a Paenibacillus clade

Lien Bosmans; Irene de Bruijn; S. Gerards; Rob Moerkens; Lore Van Looveren; Lieve Wittemans; Bart Van Calenberge; Anneleen Paeleman; Stef Van Kerckhove; Jef Rozenski; Rene de Mot; Hans Rediers; J.M. Raaijmakers; Bart Lievens

doi:10.3389/fmicb.2017.00447

Potential for biocontrol of hairy root disease by a Paenibacillus clade

Lien Bosmans, Irene de Bruijn, S. Gerards, Rob Moerkens, Lore Van Looveren, Lieve Wittemans, Bart Van Calenberge, Anneleen Paeleman, Stef Van Kerckhove, Jef Rozenski, Rene de Mot, Hans Rediers, J.M. Raaijmakers, Bart Lievens (Co-auteur)

NIOO - Microbial Ecology (ME)

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

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Rhizogenic Agrobacterium biovar 1 is the causative agent of hairy root disease (HRD) in the hydroponic cultivation of tomato and cucumber causing significant losses in marketable yield. In order to prevent and control the disease chemical disinfectants such as hydrogen peroxide or hypochlorite are generally applied to sanitize the hydroponic system and/or hydroponic solution. However, effective control of HRD sometimes requires high disinfectant doses that may have phytotoxic effects. Moreover, several of these chemicals may be converted to unwanted by-products with human health hazards. Here we explored the potential of beneficial bacteria as a sustainable means to control HRD. A large collection of diverse bacterial genera was screened for antagonistic activity against rhizogenic Agrobacterium biovar 1 using the agar overlay assay. Out of more than 130 strains tested only Paenibacillus strains showed antagonistic activity. Strikingly, phylogenetic analysis showed that antagonistic activity was restricted to a particular Paenibacillus clade, representing the species P. illinoisensis, P. pabuli, P. taichungensis, P. tundrae, P. tylopili, P. xylanexedens and P. xylanilyticus. Assessment of the spectrum of activity revealed that some strains were able to inhibit the growth of all 35 rhizogenic agrobacteria strains tested, while others were only active against part of the collection, suggesting a different mode of action. Preliminary characterization of the compounds involved in the antagonistic activity of two closely related Paenibacillus strains, tentatively identified as P. xylanexedens, revealed that they are water-soluble and have low molecular weight. Application of a combination of these strains in greenhouse conditions resulted in a significant reduction of HRD, indicating the great potential of these strains to control HRD.

Originele taal-2	Engels
Artikelnummer	447
Tijdschrift	Frontiers in Microbiology
Volume	8
Vroegere onlinedatum	2017
DOI's	https://doi.org/10.3389/fmicb.2017.00447
Status	Gepubliceerd - 2017

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10.3389/fmicb.2017.00447Licentie: CC BY

243031_Bosmans_FrontiersManuscript (002)Accepted author manuscript, 594 KBLicentie: CC BY
6257_BosmansFinal published version, 1,26 MBLicentie: CC BY

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Bosmans, L., de Bruijn, I., Gerards, S., Moerkens, R., Van Looveren, L., Wittemans, L., Van Calenberge, B., Paeleman, A., Van Kerckhove, S., Rozenski, J., de Mot, R., Rediers, H., Raaijmakers, J. M., & Lievens, B. (2017). Potential for biocontrol of hairy root disease by a Paenibacillus clade. Frontiers in Microbiology, 8, Artikel 447. https://doi.org/10.3389/fmicb.2017.00447

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title = "Potential for biocontrol of hairy root disease by a Paenibacillus clade",

abstract = "Rhizogenic Agrobacterium biovar 1 is the causative agent of hairy root disease (HRD) in the hydroponic cultivation of tomato and cucumber causing significant losses in marketable yield. In order to prevent and control the disease chemical disinfectants such as hydrogen peroxide or hypochlorite are generally applied to sanitize the hydroponic system and/or hydroponic solution. However, effective control of HRD sometimes requires high disinfectant doses that may have phytotoxic effects. Moreover, several of these chemicals may be converted to unwanted by-products with human health hazards. Here we explored the potential of beneficial bacteria as a sustainable means to control HRD. A large collection of diverse bacterial genera was screened for antagonistic activity against rhizogenic Agrobacterium biovar 1 using the agar overlay assay. Out of more than 130 strains tested only Paenibacillus strains showed antagonistic activity. Strikingly, phylogenetic analysis showed that antagonistic activity was restricted to a particular Paenibacillus clade, representing the species P. illinoisensis, P. pabuli, P. taichungensis, P. tundrae, P. tylopili, P. xylanexedens and P. xylanilyticus. Assessment of the spectrum of activity revealed that some strains were able to inhibit the growth of all 35 rhizogenic agrobacteria strains tested, while others were only active against part of the collection, suggesting a different mode of action. Preliminary characterization of the compounds involved in the antagonistic activity of two closely related Paenibacillus strains, tentatively identified as P. xylanexedens, revealed that they are water-soluble and have low molecular weight. Application of a combination of these strains in greenhouse conditions resulted in a significant reduction of HRD, indicating the great potential of these strains to control HRD.",

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author = "Lien Bosmans and {de Bruijn}, Irene and S. Gerards and Rob Moerkens and {Van Looveren}, Lore and Lieve Wittemans and {Van Calenberge}, Bart and Anneleen Paeleman and {Van Kerckhove}, Stef and Jef Rozenski and {de Mot}, Rene and Hans Rediers and J.M. Raaijmakers and Bart Lievens",

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AU - Bosmans, Lien

AU - de Bruijn, Irene

AU - Gerards, S.

AU - Moerkens, Rob

AU - Van Looveren, Lore

AU - Wittemans, Lieve

AU - Van Calenberge, Bart

AU - Paeleman, Anneleen

AU - Van Kerckhove, Stef

AU - Rozenski, Jef

AU - de Mot, Rene

AU - Rediers, Hans

AU - Raaijmakers, J.M.

AU - Lievens, Bart

N1 - 6257, ME; Data archiving: Eigendom KU Leuven

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N2 - Rhizogenic Agrobacterium biovar 1 is the causative agent of hairy root disease (HRD) in the hydroponic cultivation of tomato and cucumber causing significant losses in marketable yield. In order to prevent and control the disease chemical disinfectants such as hydrogen peroxide or hypochlorite are generally applied to sanitize the hydroponic system and/or hydroponic solution. However, effective control of HRD sometimes requires high disinfectant doses that may have phytotoxic effects. Moreover, several of these chemicals may be converted to unwanted by-products with human health hazards. Here we explored the potential of beneficial bacteria as a sustainable means to control HRD. A large collection of diverse bacterial genera was screened for antagonistic activity against rhizogenic Agrobacterium biovar 1 using the agar overlay assay. Out of more than 130 strains tested only Paenibacillus strains showed antagonistic activity. Strikingly, phylogenetic analysis showed that antagonistic activity was restricted to a particular Paenibacillus clade, representing the species P. illinoisensis, P. pabuli, P. taichungensis, P. tundrae, P. tylopili, P. xylanexedens and P. xylanilyticus. Assessment of the spectrum of activity revealed that some strains were able to inhibit the growth of all 35 rhizogenic agrobacteria strains tested, while others were only active against part of the collection, suggesting a different mode of action. Preliminary characterization of the compounds involved in the antagonistic activity of two closely related Paenibacillus strains, tentatively identified as P. xylanexedens, revealed that they are water-soluble and have low molecular weight. Application of a combination of these strains in greenhouse conditions resulted in a significant reduction of HRD, indicating the great potential of these strains to control HRD.

AB - Rhizogenic Agrobacterium biovar 1 is the causative agent of hairy root disease (HRD) in the hydroponic cultivation of tomato and cucumber causing significant losses in marketable yield. In order to prevent and control the disease chemical disinfectants such as hydrogen peroxide or hypochlorite are generally applied to sanitize the hydroponic system and/or hydroponic solution. However, effective control of HRD sometimes requires high disinfectant doses that may have phytotoxic effects. Moreover, several of these chemicals may be converted to unwanted by-products with human health hazards. Here we explored the potential of beneficial bacteria as a sustainable means to control HRD. A large collection of diverse bacterial genera was screened for antagonistic activity against rhizogenic Agrobacterium biovar 1 using the agar overlay assay. Out of more than 130 strains tested only Paenibacillus strains showed antagonistic activity. Strikingly, phylogenetic analysis showed that antagonistic activity was restricted to a particular Paenibacillus clade, representing the species P. illinoisensis, P. pabuli, P. taichungensis, P. tundrae, P. tylopili, P. xylanexedens and P. xylanilyticus. Assessment of the spectrum of activity revealed that some strains were able to inhibit the growth of all 35 rhizogenic agrobacteria strains tested, while others were only active against part of the collection, suggesting a different mode of action. Preliminary characterization of the compounds involved in the antagonistic activity of two closely related Paenibacillus strains, tentatively identified as P. xylanexedens, revealed that they are water-soluble and have low molecular weight. Application of a combination of these strains in greenhouse conditions resulted in a significant reduction of HRD, indicating the great potential of these strains to control HRD.

KW - international

U2 - 10.3389/fmicb.2017.00447

DO - 10.3389/fmicb.2017.00447

M3 - Article

SN - 1664-302X

VL - 8

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

M1 - 447

ER -

Potential for biocontrol of hairy root disease by a Paenibacillus clade

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