Premigratory and migratory neural crest cells are multipotent in vivo

Arianna Baggiolini; Sandra Varum; José María Mateos; Damiano Bettosini; Nessy John; Mario Bonalli; Urs Ziegler; Leda Dimou; Hans Clevers; Reinhard Furrer; Lukas Sommer

doi:10.1016/j.stem.2015.02.017

Premigratory and migratory neural crest cells are multipotent in vivo

Arianna Baggiolini, Sandra Varum, José María Mateos, Damiano Bettosini, Nessy John, Mario Bonalli, Urs Ziegler, Leda Dimou, Hans Clevers, Reinhard Furrer, Lukas Sommer

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

161 Citaten (Scopus)

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The neural crest (NC) is an embryonic stem/progenitor cell population that generates a diverse array of cell lineages, including peripheral neurons, myelinating Schwann cells, and melanocytes, among others. However, there is a long-standing controversy as to whether this broad developmental perspective reflects in vivo multipotency of individual NC cells or whether the NC is comprised of a heterogeneous mixture of lineage-restricted progenitors. Here, we resolve this controversy by performing in vivo fate mapping of single trunk NC cells both at premigratory and migratory stages using the R26R-Confetti mouse model. By combining quantitative clonal analyses with definitive markers of differentiation, we demonstrate that the vast majority of individual NC cells are multipotent, with only few clones contributing to single derivatives. Intriguingly, multipotency is maintained in migratory NC cells. Thus, our findings provide definitive evidence for the in vivo multipotency of both premigratory and migrating NC cells in the mouse.

Originele taal-2	Engels
Pagina's (van-tot)	314-22
Aantal pagina's	9
Tijdschrift	Cell Stem Cell
Volume	16
Nummer van het tijdschrift	3
DOI's	https://doi.org/10.1016/j.stem.2015.02.017
Status	Gepubliceerd - 05 mrt. 2015

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10.1016/j.stem.2015.02.017

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title = "Premigratory and migratory neural crest cells are multipotent in vivo",

abstract = "The neural crest (NC) is an embryonic stem/progenitor cell population that generates a diverse array of cell lineages, including peripheral neurons, myelinating Schwann cells, and melanocytes, among others. However, there is a long-standing controversy as to whether this broad developmental perspective reflects in vivo multipotency of individual NC cells or whether the NC is comprised of a heterogeneous mixture of lineage-restricted progenitors. Here, we resolve this controversy by performing in vivo fate mapping of single trunk NC cells both at premigratory and migratory stages using the R26R-Confetti mouse model. By combining quantitative clonal analyses with definitive markers of differentiation, we demonstrate that the vast majority of individual NC cells are multipotent, with only few clones contributing to single derivatives. Intriguingly, multipotency is maintained in migratory NC cells. Thus, our findings provide definitive evidence for the in vivo multipotency of both premigratory and migrating NC cells in the mouse.",

author = "Arianna Baggiolini and Sandra Varum and Mateos, {Jos{\'e} Mar{\'i}a} and Damiano Bettosini and Nessy John and Mario Bonalli and Urs Ziegler and Leda Dimou and Hans Clevers and Reinhard Furrer and Lukas Sommer",

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AU - Baggiolini, Arianna

AU - Varum, Sandra

AU - Mateos, José María

AU - Bettosini, Damiano

AU - John, Nessy

AU - Bonalli, Mario

AU - Ziegler, Urs

AU - Dimou, Leda

AU - Clevers, Hans

AU - Furrer, Reinhard

AU - Sommer, Lukas

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N2 - The neural crest (NC) is an embryonic stem/progenitor cell population that generates a diverse array of cell lineages, including peripheral neurons, myelinating Schwann cells, and melanocytes, among others. However, there is a long-standing controversy as to whether this broad developmental perspective reflects in vivo multipotency of individual NC cells or whether the NC is comprised of a heterogeneous mixture of lineage-restricted progenitors. Here, we resolve this controversy by performing in vivo fate mapping of single trunk NC cells both at premigratory and migratory stages using the R26R-Confetti mouse model. By combining quantitative clonal analyses with definitive markers of differentiation, we demonstrate that the vast majority of individual NC cells are multipotent, with only few clones contributing to single derivatives. Intriguingly, multipotency is maintained in migratory NC cells. Thus, our findings provide definitive evidence for the in vivo multipotency of both premigratory and migrating NC cells in the mouse.

AB - The neural crest (NC) is an embryonic stem/progenitor cell population that generates a diverse array of cell lineages, including peripheral neurons, myelinating Schwann cells, and melanocytes, among others. However, there is a long-standing controversy as to whether this broad developmental perspective reflects in vivo multipotency of individual NC cells or whether the NC is comprised of a heterogeneous mixture of lineage-restricted progenitors. Here, we resolve this controversy by performing in vivo fate mapping of single trunk NC cells both at premigratory and migratory stages using the R26R-Confetti mouse model. By combining quantitative clonal analyses with definitive markers of differentiation, we demonstrate that the vast majority of individual NC cells are multipotent, with only few clones contributing to single derivatives. Intriguingly, multipotency is maintained in migratory NC cells. Thus, our findings provide definitive evidence for the in vivo multipotency of both premigratory and migrating NC cells in the mouse.

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DO - 10.1016/j.stem.2015.02.017

M3 - Article

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JO - Cell Stem Cell

JF - Cell Stem Cell

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Premigratory and migratory neural crest cells are multipotent in vivo

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