Prevalence and antifungal susceptibility of Sporothrix species in Jiangxi, central China

TY - JOUR

T1 - Prevalence and antifungal susceptibility of Sporothrix species in Jiangxi, central China

AU - Li, Jie

AU - Zhan, Ping

AU - Jiang, Qing

AU - Gao, Yangmin

AU - Jin, Yun

AU - Zhang, Lei

AU - Luo, Yunpeng

AU - Fan, Xinyi

AU - Sun, Jiufeng

AU - de Hoog, Sybren

N1 - © The Author(s) 2019. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Sporotrichosis is a subcutaneous mycosis caused by traumatic inoculation of pathogenic Sporothrix species. Until recently, Sporothrix globosa was considered as the unique Chinese species causing this disorder. In the present study, 33 clinical Sporothrix strains isolated from Jiangxi, China, were classified and antifungal susceptibility for each strain was determined. Thirteen S. globosa strains and 20 S. schenckii strains were identified by morphology and by multilocus analysis using rDNA ITS, CAL, and EF1α (i.e., internal transcribed spacer, calmodulin and elongation factor-1α). In vitro antifungal susceptibility testing of yeast phases indicated that itraconazole, terbinafine, and posaconazole were most effective against both species, followed by amphotericin B and voriconazole, while fluconazole, 5-fluorocytosine had low efficacy with high MICs. Co-occurrence of S. schenckii and S. globosa in central China may indicate different routes of transmission in this area.

AB - Sporotrichosis is a subcutaneous mycosis caused by traumatic inoculation of pathogenic Sporothrix species. Until recently, Sporothrix globosa was considered as the unique Chinese species causing this disorder. In the present study, 33 clinical Sporothrix strains isolated from Jiangxi, China, were classified and antifungal susceptibility for each strain was determined. Thirteen S. globosa strains and 20 S. schenckii strains were identified by morphology and by multilocus analysis using rDNA ITS, CAL, and EF1α (i.e., internal transcribed spacer, calmodulin and elongation factor-1α). In vitro antifungal susceptibility testing of yeast phases indicated that itraconazole, terbinafine, and posaconazole were most effective against both species, followed by amphotericin B and voriconazole, while fluconazole, 5-fluorocytosine had low efficacy with high MICs. Co-occurrence of S. schenckii and S. globosa in central China may indicate different routes of transmission in this area.

KW - Adolescent

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Antifungal Agents/pharmacology

KW - Child

KW - Child, Preschool

KW - China/epidemiology

KW - DNA, Ribosomal Spacer/genetics

KW - Female

KW - Genotype

KW - Humans

KW - Male

KW - Microbial Sensitivity Tests

KW - Microscopy

KW - Middle Aged

KW - Multilocus Sequence Typing

KW - Peptide Elongation Factor 1/genetics

KW - Prevalence

KW - Sporothrix/classification

KW - Sporotrichosis/epidemiology

KW - Young Adult

U2 - 10.1093/mmy/myy163

DO - 10.1093/mmy/myy163

M3 - Article

C2 - 30657948

SN - 1369-3786

VL - 57

SP - 954

EP - 961

JO - Medical Mycology

JF - Medical Mycology

IS - 8

ER -