Primers for 22 candidate genes for ecological adaptations in Brassicaceae

H. Kuittinen, M. Aguadé, D. Charlesworth, A.D.E. Haan, B. Lauga, T. Mitchell-Olds, S. Oikarinen, S. Ramos-Onsins, B. Stranger, P.H. Van Tienderen, O. Savolainen

    Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

    Samenvatting

    There is an increasing interest in direct screening of polymorphisms at candidate loci to associate them with adaptations in natural situations. We report primers that amplify regions at 22 putatively orthologous functional loci in the family Brassicaceae: Arabidopsis thaliana, its two wild outcrossing relatives, and Brassica oleracea. Four groups of genes were known to have specific functions in the model species A. thaliana in ecologically important traits: timing of reproduction; metabolism of secondary compounds; defence against pathogens or light perception. In addition, genes of a fifth group were selected with no consideration of their function, as reference loci. [KEYWORDS: Arabidopsis; candidate genes; defence-genes; flowering time; molecular adaptation; phenylpropanoid pathway; phytochromes]
    Originele taal-2Engels
    Pagina's (van-tot)258-262
    TijdschriftMolecular Ecology Notes
    Volume2
    Nummer van het tijdschrift3
    DOI's
    StatusGepubliceerd - 2002

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