Quantification of Tyrosine Hydroxylase and ErbB4 in the Locus Coeruleus of Mood Disorder Patients Using a Multispectral Method to Prevent Interference with Immunocytochemical Signals by Neuromelanin.

Lei Guo; Jochem Stormmesand; Zheng Fang; Qingbin Zhu; Rawien Balesar; Joop van Heerikhuize; Arja Sluiter; Dick Swaab; Ai-Min Bao

doi:10.1007/s12264-019-00339-y

Quantification of Tyrosine Hydroxylase and ErbB4 in the Locus Coeruleus of Mood Disorder Patients Using a Multispectral Method to Prevent Interference with Immunocytochemical Signals by Neuromelanin.

Lei Guo, Jochem Stormmesand, Zheng Fang, Qingbin Zhu, Rawien Balesar, Joop van Heerikhuize, Arja Sluiter, Dick Swaab, Ai-Min Bao

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The locus coeruleus (LC) has been studied in major depressive disorder (MDD) and bipolar disorder (BD). A major problem of immunocytochemical studies in the human LC is interference with the staining of the immunocytochemical end-product by the omnipresent natural brown pigment neuromelanin. Here, we used a multispectral method to untangle the two colors: blue immunocytochemical staining and brown neuromelanin. We found significantly increased tyrosine hydroxylase (TH) in the LC of MDD patients-thus validating the method-but not in BD patients, and we did not find significant changes in the receptor tyrosine-protein kinase ErbB4 in the LC in MDD or BD patients. We observed clear co-localization of ErbB4, TH, and neuromelanin in the LC neurons. The different stress-related molecular changes in the LC may contribute to the different clinical symptoms in MDD and BD.

Originele taal-2	Engels
Pagina's (van-tot)	205-215
Tijdschrift	Neuroscience Bulletin
Volume	35
DOI's	https://doi.org/10.1007/s12264-019-00339-y
Status	Gepubliceerd - jan. 2019

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Guo, L., Stormmesand, J., Fang, Z., Zhu, Q., Balesar, R., van Heerikhuize, J., Sluiter, A., Swaab, D., & Bao, A.-M. (2019). Quantification of Tyrosine Hydroxylase and ErbB4 in the Locus Coeruleus of Mood Disorder Patients Using a Multispectral Method to Prevent Interference with Immunocytochemical Signals by Neuromelanin. Neuroscience Bulletin, 35, 205-215. https://doi.org/10.1007/s12264-019-00339-y

@article{1c2b533e450e4fb197336581114aaa9c,

title = "Quantification of Tyrosine Hydroxylase and ErbB4 in the Locus Coeruleus of Mood Disorder Patients Using a Multispectral Method to Prevent Interference with Immunocytochemical Signals by Neuromelanin.",

abstract = "The locus coeruleus (LC) has been studied in major depressive disorder (MDD) and bipolar disorder (BD). A major problem of immunocytochemical studies in the human LC is interference with the staining of the immunocytochemical end-product by the omnipresent natural brown pigment neuromelanin. Here, we used a multispectral method to untangle the two colors: blue immunocytochemical staining and brown neuromelanin. We found significantly increased tyrosine hydroxylase (TH) in the LC of MDD patients-thus validating the method-but not in BD patients, and we did not find significant changes in the receptor tyrosine-protein kinase ErbB4 in the LC in MDD or BD patients. We observed clear co-localization of ErbB4, TH, and neuromelanin in the LC neurons. The different stress-related molecular changes in the LC may contribute to the different clinical symptoms in MDD and BD.",

author = "Lei Guo and Jochem Stormmesand and Zheng Fang and Qingbin Zhu and Rawien Balesar and {van Heerikhuize}, Joop and Arja Sluiter and Dick Swaab and Ai-Min Bao",

year = "2019",

month = jan,

doi = "10.1007/s12264-019-00339-y",

language = "English",

volume = "35",

pages = "205--215",

journal = "Neuroscience Bulletin",

issn = "1673-7067",

publisher = "Science Press",

}

Guo, L, Stormmesand, J, Fang, Z, Zhu, Q, Balesar, R , van Heerikhuize, J , Sluiter, A , Swaab, D & Bao, A-M 2019, 'Quantification of Tyrosine Hydroxylase and ErbB4 in the Locus Coeruleus of Mood Disorder Patients Using a Multispectral Method to Prevent Interference with Immunocytochemical Signals by Neuromelanin.', Neuroscience Bulletin, vol. 35, blz. 205-215. https://doi.org/10.1007/s12264-019-00339-y

Quantification of Tyrosine Hydroxylase and ErbB4 in the Locus Coeruleus of Mood Disorder Patients Using a Multispectral Method to Prevent Interference with Immunocytochemical Signals by Neuromelanin. / Guo, Lei; Stormmesand, Jochem; Fang, Zheng et al.
In: Neuroscience Bulletin, Vol. 35, 01.2019, blz. 205-215.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

TY - JOUR

T1 - Quantification of Tyrosine Hydroxylase and ErbB4 in the Locus Coeruleus of Mood Disorder Patients Using a Multispectral Method to Prevent Interference with Immunocytochemical Signals by Neuromelanin.

AU - Guo, Lei

AU - Stormmesand, Jochem

AU - Fang, Zheng

AU - Zhu, Qingbin

AU - Balesar, Rawien

AU - van Heerikhuize, Joop

AU - Sluiter, Arja

AU - Swaab, Dick

AU - Bao, Ai-Min

PY - 2019/1

Y1 - 2019/1

N2 - The locus coeruleus (LC) has been studied in major depressive disorder (MDD) and bipolar disorder (BD). A major problem of immunocytochemical studies in the human LC is interference with the staining of the immunocytochemical end-product by the omnipresent natural brown pigment neuromelanin. Here, we used a multispectral method to untangle the two colors: blue immunocytochemical staining and brown neuromelanin. We found significantly increased tyrosine hydroxylase (TH) in the LC of MDD patients-thus validating the method-but not in BD patients, and we did not find significant changes in the receptor tyrosine-protein kinase ErbB4 in the LC in MDD or BD patients. We observed clear co-localization of ErbB4, TH, and neuromelanin in the LC neurons. The different stress-related molecular changes in the LC may contribute to the different clinical symptoms in MDD and BD.

AB - The locus coeruleus (LC) has been studied in major depressive disorder (MDD) and bipolar disorder (BD). A major problem of immunocytochemical studies in the human LC is interference with the staining of the immunocytochemical end-product by the omnipresent natural brown pigment neuromelanin. Here, we used a multispectral method to untangle the two colors: blue immunocytochemical staining and brown neuromelanin. We found significantly increased tyrosine hydroxylase (TH) in the LC of MDD patients-thus validating the method-but not in BD patients, and we did not find significant changes in the receptor tyrosine-protein kinase ErbB4 in the LC in MDD or BD patients. We observed clear co-localization of ErbB4, TH, and neuromelanin in the LC neurons. The different stress-related molecular changes in the LC may contribute to the different clinical symptoms in MDD and BD.

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DO - 10.1007/s12264-019-00339-y

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SN - 1673-7067

VL - 35

SP - 205

EP - 215

JO - Neuroscience Bulletin

JF - Neuroscience Bulletin

ER -

Quantification of Tyrosine Hydroxylase and ErbB4 in the Locus Coeruleus of Mood Disorder Patients Using a Multispectral Method to Prevent Interference with Immunocytochemical Signals by Neuromelanin.

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