Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS

Abdullah Ms Al-Hatmi; Anne-Cécile Normand; Anne D van Diepeningen; Marijke Hendrickx; G Sybren de Hoog; Renaud Piarroux

doi:10.2217/fmb.15.108

Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS

Abdullah Ms Al-Hatmi, Anne-Cécile Normand, Anne D van Diepeningen, Marijke Hendrickx, G Sybren de Hoog, Renaud Piarroux

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

32 Citaten (Scopus)

Samenvatting

AIM: To develop the matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) method for identification of Fusarium species within Fusarium fujikuroi complex for use in clinical microbiology laboratories.

MATERIALS & METHODS: A total of 24 reference and 60 clinical and environmental isolates belonging to the F. fujikuroi complex were subjected to MALDI-TOF MS identification. Protein extracts of Fusarium isolates were obtained using formic acid extraction. Multilocus sequence analysis was used as a gold standard.

RESULTS: The MALDI-TOF MS Biotyper correctly identified 93.6% of the strains down to the species level, while the remaining isolates (6.4%) were identified at the genus level when using scores of ≥ 2.0 as cut-off values. Correct identification was obtained despite large intraspecific heterogeneities in MALDI-TOF spectra.

CONCLUSION: MALDI-TOF MS is a rapid identification tool for the recognition of species within F. fujikuroi complex, provided a database is available.

Originele taal-2	Engels
Pagina's (van-tot)	1939-52
Aantal pagina's	14
Tijdschrift	Future Microbiology
Volume	10
DOI's	https://doi.org/10.2217/fmb.15.108
Status	Gepubliceerd - dec. 2015

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10.2217/fmb.15.108

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title = "Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS",

abstract = "AIM: To develop the matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) method for identification of Fusarium species within Fusarium fujikuroi complex for use in clinical microbiology laboratories.MATERIALS & METHODS: A total of 24 reference and 60 clinical and environmental isolates belonging to the F. fujikuroi complex were subjected to MALDI-TOF MS identification. Protein extracts of Fusarium isolates were obtained using formic acid extraction. Multilocus sequence analysis was used as a gold standard.RESULTS: The MALDI-TOF MS Biotyper correctly identified 93.6% of the strains down to the species level, while the remaining isolates (6.4%) were identified at the genus level when using scores of ≥ 2.0 as cut-off values. Correct identification was obtained despite large intraspecific heterogeneities in MALDI-TOF spectra.CONCLUSION: MALDI-TOF MS is a rapid identification tool for the recognition of species within F. fujikuroi complex, provided a database is available.",

author = "Al-Hatmi, {Abdullah Ms} and Anne-C{\'e}cile Normand and {van Diepeningen}, {Anne D} and Marijke Hendrickx and {de Hoog}, {G Sybren} and Renaud Piarroux",

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doi = "10.2217/fmb.15.108",

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T1 - Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS

AU - Al-Hatmi, Abdullah Ms

AU - Normand, Anne-Cécile

AU - van Diepeningen, Anne D

AU - Hendrickx, Marijke

AU - de Hoog, G Sybren

AU - Piarroux, Renaud

PY - 2015/12

Y1 - 2015/12

N2 - AIM: To develop the matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) method for identification of Fusarium species within Fusarium fujikuroi complex for use in clinical microbiology laboratories.MATERIALS & METHODS: A total of 24 reference and 60 clinical and environmental isolates belonging to the F. fujikuroi complex were subjected to MALDI-TOF MS identification. Protein extracts of Fusarium isolates were obtained using formic acid extraction. Multilocus sequence analysis was used as a gold standard.RESULTS: The MALDI-TOF MS Biotyper correctly identified 93.6% of the strains down to the species level, while the remaining isolates (6.4%) were identified at the genus level when using scores of ≥ 2.0 as cut-off values. Correct identification was obtained despite large intraspecific heterogeneities in MALDI-TOF spectra.CONCLUSION: MALDI-TOF MS is a rapid identification tool for the recognition of species within F. fujikuroi complex, provided a database is available.

AB - AIM: To develop the matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) method for identification of Fusarium species within Fusarium fujikuroi complex for use in clinical microbiology laboratories.MATERIALS & METHODS: A total of 24 reference and 60 clinical and environmental isolates belonging to the F. fujikuroi complex were subjected to MALDI-TOF MS identification. Protein extracts of Fusarium isolates were obtained using formic acid extraction. Multilocus sequence analysis was used as a gold standard.RESULTS: The MALDI-TOF MS Biotyper correctly identified 93.6% of the strains down to the species level, while the remaining isolates (6.4%) were identified at the genus level when using scores of ≥ 2.0 as cut-off values. Correct identification was obtained despite large intraspecific heterogeneities in MALDI-TOF spectra.CONCLUSION: MALDI-TOF MS is a rapid identification tool for the recognition of species within F. fujikuroi complex, provided a database is available.

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