Rapid screening for genotypes as possible markers of virulence in the neurotropic black yeast Exophiala dermatiddis using PCR-RFLP

TY - JOUR

T1 - Rapid screening for genotypes as possible markers of virulence in the neurotropic black yeast Exophiala dermatiddis using PCR-RFLP

AU - Sudhadham, M.

AU - de Hoog, G.S.

AU - Menken, S.B.J.

AU - Gerrits Van Den Ende, A. H.

AU - Sihanonth, P.

N1 - Reporting year: 2010

PY - 2010

Y1 - 2010

N2 - A simple method for fungal genotype screening was developed for the black yeast Exophiala dermatitidis based on RFLP of ribosomal ITS regions currently used as potential virulence markers. In a study set of 502 strains of the species, two main genotypes were recognized. Only 0.97% of lanes were difficult to interpret as they did not clearly present one of the expected genotypes. Twenty strains were deviating and proved to be E. spinifera after sequencing. Eight common. related species (based on SSU data) with clinical significance yielded different patterns with TaqI digestion, and thus the method is also usable for routine diagnostics. (C) 2009 Published by Elsevier B.V.

AB - A simple method for fungal genotype screening was developed for the black yeast Exophiala dermatitidis based on RFLP of ribosomal ITS regions currently used as potential virulence markers. In a study set of 502 strains of the species, two main genotypes were recognized. Only 0.97% of lanes were difficult to interpret as they did not clearly present one of the expected genotypes. Twenty strains were deviating and proved to be E. spinifera after sequencing. Eight common. related species (based on SSU data) with clinical significance yielded different patterns with TaqI digestion, and thus the method is also usable for routine diagnostics. (C) 2009 Published by Elsevier B.V.

U2 - 10.1016/j.mimet.2009.11.007

DO - 10.1016/j.mimet.2009.11.007

M3 - Article

SN - 0167-7012

VL - 80

SP - 138

EP - 142

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 2

ER -