Regional variations in stiffness in live mouse brain tissue determined by depth-controlled indentation mapping

TY - JOUR

T1 - Regional variations in stiffness in live mouse brain tissue determined by depth-controlled indentation mapping

AU - Antonovaite, Nelda

AU - Beekmans, Steven V

AU - Hol, Elly M

AU - Wadman, Wytse J

AU - Iannuzzi, Davide

PY - 2018

Y1 - 2018

N2 - The mechanical properties of brain tissue play a pivotal role in neurodevelopment and neurological disorders. Yet, at present, there is no consensus on how the different structural parts of the tissue contribute to its stiffness variations. Here, we have gathered depth-controlled indentation viscoelasticity maps of the hippocampus of acute horizontal live mouse brain slices. Our results confirm the highly viscoelestic nature of brain tissue. We further show that the mechanical properties are non-uniform and at least related to differences in morphological composition. Interestingly, areas with higher nuclear density appear to be softer than areas with lower nuclear density.

AB - The mechanical properties of brain tissue play a pivotal role in neurodevelopment and neurological disorders. Yet, at present, there is no consensus on how the different structural parts of the tissue contribute to its stiffness variations. Here, we have gathered depth-controlled indentation viscoelasticity maps of the hippocampus of acute horizontal live mouse brain slices. Our results confirm the highly viscoelestic nature of brain tissue. We further show that the mechanical properties are non-uniform and at least related to differences in morphological composition. Interestingly, areas with higher nuclear density appear to be softer than areas with lower nuclear density.

KW - Journal Article

U2 - 10.1038/s41598-018-31035-y

DO - 10.1038/s41598-018-31035-y

M3 - Article

C2 - 30131608

SN - 2045-2322

VL - 8

SP - 12517

JO - Scientific Reports

JF - Scientific Reports

IS - 1

ER -