Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts

TY - JOUR

T1 - Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts

AU - Knipscheer, P.

AU - Raschle, M.

AU - Scharer, O.D.

AU - Walter, J.C.

N1 - Reporting year: 2012

PY - 2012

Y1 - 2012

N2 - Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.

AB - Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.

U2 - 10.1007/978-1-61779-998-3_16

DO - 10.1007/978-1-61779-998-3_16

M3 - Article

SN - 1064-3745

VL - 920

SP - 221

EP - 243

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -