Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts

P. Knipscheer, M. Raschle, O.D. Scharer, J.C. Walter

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.
Originele taal-2Engels
Pagina's (van-tot)221-243
TijdschriftMethods in Molecular Biology
Volume920
DOI's
StatusGepubliceerd - 2012

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