Response of the sulfate-reducing community to the re-establishment of estuarine conditions in two contrasting soils: a mesocosm approach

TY - JOUR

T1 - Response of the sulfate-reducing community to the re-establishment of estuarine conditions in two contrasting soils: a mesocosm approach

AU - Miletto, M.

AU - Loeb, R.

AU - Antheunisse, A.M.

AU - Bodelier, P.L.E.

AU - Laanbroek, H.J.

N1 - Reporting year: 2010 Metis note: 4645;CL; ME; file:///L:\EndnoteDatabases\NIOOPUB\pdfs\PDFS2010\Miletto_ea_4645.pdf

PY - 2010

Y1 - 2010

N2 - We studied the response of the sulfate-reducing prokaryote (SRP) communities to the experimental variation of salinity and tide in an outdoor mesocosm setup. Intact soil monoliths were collected at two areas of the Haringvliet lagoon (The Netherlands): one sampling location consisted of agricultural grassland, drained and fertilized for at least the last century; the other of a freshwater marshland with more recent sea influence. Two factors, i.e., “salinity” (freshwater/oligohaline) and “tide” (nontidal/tidal), were tested in a full-factorial design. Soil samples were collected after 5 months (June–October). Dissimilatory (bi)sulfite reductase β subunit-based denaturing gradient gel electrophoresis (dsrB-DGGE) analysis revealed that the SRP community composition in the agricultural grassland and in the freshwater marshland was represented mainly by microorganisms related to the Desulfobulbaceae and the Desulfobacteraceae, respectively. Desulfovibrio-related dsrB were detected only in the tidal treatments; Desulfomonile-related dsrB occurrence was related to the presence of oligohaline conditions. Treatments did have an effect on the overall SRP community composition of both soils, but not on the sulfate depletion rates in sulfate-amended anoxic slurry incubations. However, initiation of sulfate reduction upon sulfate addition was clearly different between the two soils.

AB - We studied the response of the sulfate-reducing prokaryote (SRP) communities to the experimental variation of salinity and tide in an outdoor mesocosm setup. Intact soil monoliths were collected at two areas of the Haringvliet lagoon (The Netherlands): one sampling location consisted of agricultural grassland, drained and fertilized for at least the last century; the other of a freshwater marshland with more recent sea influence. Two factors, i.e., “salinity” (freshwater/oligohaline) and “tide” (nontidal/tidal), were tested in a full-factorial design. Soil samples were collected after 5 months (June–October). Dissimilatory (bi)sulfite reductase β subunit-based denaturing gradient gel electrophoresis (dsrB-DGGE) analysis revealed that the SRP community composition in the agricultural grassland and in the freshwater marshland was represented mainly by microorganisms related to the Desulfobulbaceae and the Desulfobacteraceae, respectively. Desulfovibrio-related dsrB were detected only in the tidal treatments; Desulfomonile-related dsrB occurrence was related to the presence of oligohaline conditions. Treatments did have an effect on the overall SRP community composition of both soils, but not on the sulfate depletion rates in sulfate-amended anoxic slurry incubations. However, initiation of sulfate reduction upon sulfate addition was clearly different between the two soils.

U2 - 10.1007/s00248-009-9614-9

DO - 10.1007/s00248-009-9614-9

M3 - Article

SN - 0095-3628

VL - 59

SP - 109

EP - 120

JO - Microbial Ecology

JF - Microbial Ecology

IS - 1

ER -