Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues

A.K. Lagendijk, J.D. Moulton, J. Bakkers

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

22 Citaten (Scopus)

Samenvatting

Non-coding microRNA (miRNA) molecules bind their target mRNAs and thereby modulate the amount of protein produced. To understand the significance of a potential miRNA-mRNA interaction, temporal and spatial information on miRNA and mRNA expression is essential. Here, we provide a detailed protocol for miRNA whole mount in situ hybridization. We introduce the use of Morpholino based oligos as antisense probes for miRNA detection, in addition to the current "gold standard" locked nucleic acid (LNA) probes. Furthermore we have modified existing miRNA in situ protocols thereby improving both sensitivity and resolution of miRNA visualization in whole zebrafish embryos and adult tissues.
Originele taal-2Engels
Pagina's (van-tot)566-569
TijdschriftBiology Open
Volume1
Nummer van het tijdschrift6
DOI's
StatusGepubliceerd - 2012

Vingerafdruk

Duik in de onderzoeksthema's van 'Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues'. Samen vormen ze een unieke vingerafdruk.

Citeer dit