Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues

TY - JOUR

T1 - Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues

AU - Lagendijk, A.K.

AU - Moulton, J.D.

AU - Bakkers, J.

N1 - Reporting year: 2012

PY - 2012

Y1 - 2012

N2 - Non-coding microRNA (miRNA) molecules bind their target mRNAs and thereby modulate the amount of protein produced. To understand the significance of a potential miRNA-mRNA interaction, temporal and spatial information on miRNA and mRNA expression is essential. Here, we provide a detailed protocol for miRNA whole mount in situ hybridization. We introduce the use of Morpholino based oligos as antisense probes for miRNA detection, in addition to the current "gold standard" locked nucleic acid (LNA) probes. Furthermore we have modified existing miRNA in situ protocols thereby improving both sensitivity and resolution of miRNA visualization in whole zebrafish embryos and adult tissues.

AB - Non-coding microRNA (miRNA) molecules bind their target mRNAs and thereby modulate the amount of protein produced. To understand the significance of a potential miRNA-mRNA interaction, temporal and spatial information on miRNA and mRNA expression is essential. Here, we provide a detailed protocol for miRNA whole mount in situ hybridization. We introduce the use of Morpholino based oligos as antisense probes for miRNA detection, in addition to the current "gold standard" locked nucleic acid (LNA) probes. Furthermore we have modified existing miRNA in situ protocols thereby improving both sensitivity and resolution of miRNA visualization in whole zebrafish embryos and adult tissues.

U2 - 10.1242/bio.2012810

DO - 10.1242/bio.2012810

M3 - Article

SN - 2046-6390

VL - 1

SP - 566

EP - 569

JO - Biology Open

JF - Biology Open

IS - 6

ER -