Samenvatting
Regulatory DNA elements can control the expression of distant genes via physical interactions. Here we present a cost-effective methodology and computational analysis pipeline for robust characterization of the physical organization around selected promoters and other functional elements using chromosome conformation capture combined with high-throughput sequencing (4C-seq). Our approach can be multiplexed and routinely integrated with other functional genomics assays to facilitate physical characterization of gene regulation.
Originele taal-2 | Engels |
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Pagina's (van-tot) | 969-972 |
Tijdschrift | Nature Methods |
Volume | 9 |
Nummer van het tijdschrift | 10 |
DOI's | |
Status | Gepubliceerd - 2012 |