Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish

I. Geudens, R. Herpers, K. Hermans, I. Segura, C. Ruiz de Almodovar, J. Bussmann, F. De Smet, W. Vandevelde, B.M. Hogan, A. Siekmann, F. Claes, J. Moore, A.S. Pistocchi, S. Loges, M. Mazzone, G. Mariggi, F. Bruyere, F. Cotelli, D. Kerjaschki, A. NoelJ.M. Foidart, H. Gerhardt, A. Ny, T. Langenberg, N. Lawson, H.J. Duckers, S. Schulte-Merker, P. Carmeliet, M. Dewerchin

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review


OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.
Originele taal-2Engels
Pagina's (van-tot)1695-1702
TijdschriftArtheriosclerosis, Thrombosis and Vascular Biology
Nummer van het tijdschrift9
StatusGepubliceerd - 2010


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