Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish

TY - JOUR

T1 - Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish

AU - Geudens, I.

AU - Herpers, R.

AU - Hermans, K.

AU - Segura, I.

AU - Ruiz de Almodovar, C.

AU - Bussmann, J.

AU - De Smet, F.

AU - Vandevelde, W.

AU - Hogan, B.M.

AU - Siekmann, A.

AU - Claes, F.

AU - Moore, J.

AU - Pistocchi, A.S.

AU - Loges, S.

AU - Mazzone, M.

AU - Mariggi, G.

AU - Bruyere, F.

AU - Cotelli, F.

AU - Kerjaschki, D.

AU - Noel, A.

AU - Foidart, J.M.

AU - Gerhardt, H.

AU - Ny, A.

AU - Langenberg, T.

AU - Lawson, N.

AU - Duckers, H.J.

AU - Schulte-Merker, S.

AU - Carmeliet, P.

AU - Dewerchin, M.

N1 - Reporting year: 2010

PY - 2010

Y1 - 2010

N2 - OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.

AB - OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.

U2 - 10.1161/ATVBAHA.110.203034

DO - 10.1161/ATVBAHA.110.203034

M3 - Article

SN - 1079-5642

VL - 30

SP - 1695

EP - 1702

JO - Artheriosclerosis, Thrombosis and Vascular Biology

JF - Artheriosclerosis, Thrombosis and Vascular Biology

IS - 9

ER -