Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.

T. Sato; R.G.J. Vries; H.J.G. Snippert; M.L. van de Wetering; N. Barker; D.E. Stange; J.H. van Es; A. Abo; P. Kujala; P.J. Peters; H. Clevers

doi:10.1038/nature07935

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.

T. Sato, R.G.J. Vries, H.J.G. Snippert, M.L. van de Wetering, N. Barker, D.E. Stange, J.H. van Es, A. Abo, P. Kujala, P.J. Peters, H. Clevers

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

5390 Citaten (Scopus)

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The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while simultanously generating villus-like epithelial domains in which all differentiated cell types are present. Single sorted Lgr5(+) stem cells can also initiate these cryptvillus organoids. Tracing experiments indicate that the Lgr5(+) stem-cell hierarchy is maintained in organoids. We conclude that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.

Originele taal-2	Engels
Pagina's (van-tot)	262-265
Tijdschrift	Nature
Volume	459
Nummer van het tijdschrift	7244
DOI's	https://doi.org/10.1038/nature07935
Status	Gepubliceerd - 2009

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title = "Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.",

abstract = "The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while simultanously generating villus-like epithelial domains in which all differentiated cell types are present. Single sorted Lgr5(+) stem cells can also initiate these cryptvillus organoids. Tracing experiments indicate that the Lgr5(+) stem-cell hierarchy is maintained in organoids. We conclude that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.",

author = "T. Sato and R.G.J. Vries and H.J.G. Snippert and {van de Wetering}, M.L. and N. Barker and D.E. Stange and {van Es}, J.H. and A. Abo and P. Kujala and P.J. Peters and H. Clevers",

note = "Reporting year: 2009",

year = "2009",

doi = "10.1038/nature07935",

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T1 - Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.

AU - Sato, T.

AU - Vries, R.G.J.

AU - Snippert, H.J.G.

AU - van de Wetering, M.L.

AU - Barker, N.

AU - Stange, D.E.

AU - van Es, J.H.

AU - Abo, A.

AU - Kujala, P.

AU - Peters, P.J.

AU - Clevers, H.

N1 - Reporting year: 2009

PY - 2009

Y1 - 2009

N2 - The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while simultanously generating villus-like epithelial domains in which all differentiated cell types are present. Single sorted Lgr5(+) stem cells can also initiate these cryptvillus organoids. Tracing experiments indicate that the Lgr5(+) stem-cell hierarchy is maintained in organoids. We conclude that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.

AB - The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while simultanously generating villus-like epithelial domains in which all differentiated cell types are present. Single sorted Lgr5(+) stem cells can also initiate these cryptvillus organoids. Tracing experiments indicate that the Lgr5(+) stem-cell hierarchy is maintained in organoids. We conclude that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.

U2 - 10.1038/nature07935

DO - 10.1038/nature07935

M3 - Article

SN - 0028-0836

VL - 459

SP - 262

EP - 265

JO - Nature

JF - Nature

IS - 7244

ER -

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.

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