Spatial transcriptomics of the nematode Caenorhabditis elegans using RNA tomography

TY - JOUR

T1 - Spatial transcriptomics of the nematode Caenorhabditis elegans using RNA tomography

AU - Schild, Erik S

AU - Mars, Jonas

AU - Ebbing, Annabel

AU - Vivié, Judith

AU - Betist, Marco

AU - Korswagen, Hendrik C

N1 - © 2021 The Authors.

PY - 2021/6/18

Y1 - 2021/6/18

N2 - RNA tomography or tomo-seq combines mRNA sequencing and cryo-sectioning to spatially resolve gene expression. We have adapted this method for the nematode Caenorhabditis elegans to generate anteroposterior gene expression maps at near-cellular resolution. Here, we provide a detailed overview of the method and present two approaches: one that includes RNA isolation for maximum sensitivity and one that is suitable for partial automatization and is therefore less time-consuming. For complete details on the use and execution of this protocol, please refer to Ebbing et al. (2018).

AB - RNA tomography or tomo-seq combines mRNA sequencing and cryo-sectioning to spatially resolve gene expression. We have adapted this method for the nematode Caenorhabditis elegans to generate anteroposterior gene expression maps at near-cellular resolution. Here, we provide a detailed overview of the method and present two approaches: one that includes RNA isolation for maximum sensitivity and one that is suitable for partial automatization and is therefore less time-consuming. For complete details on the use and execution of this protocol, please refer to Ebbing et al. (2018).

U2 - 10.1016/j.xpro.2021.100411

DO - 10.1016/j.xpro.2021.100411

M3 - Article

C2 - 33870220

SN - 2666-1667

VL - 2

SP - 100411

JO - STAR protocols

JF - STAR protocols

IS - 2

ER -