Spatially resolved transcriptomics and beyond

TY - JOUR

T1 - Spatially resolved transcriptomics and beyond

AU - Crosetto, Nicola

AU - Bienko, Magda

AU - van Oudenaarden, Alexander

PY - 2015/1

Y1 - 2015/1

N2 - Considerable progress in sequencing technologies makes it now possible to study the genomic and transcriptomic landscape of single cells. However, to better understand the complexity of multicellular organisms, we must devise ways to perform high-throughput measurements while preserving spatial information about the tissue context or subcellular localization of analysed nucleic acids. In this Innovation article, we summarize pioneering technologies that enable spatially resolved transcriptomics and discuss how these methods have the potential to extend beyond transcriptomics to encompass spatially resolved genomics, proteomics and possibly other omic disciplines.

AB - Considerable progress in sequencing technologies makes it now possible to study the genomic and transcriptomic landscape of single cells. However, to better understand the complexity of multicellular organisms, we must devise ways to perform high-throughput measurements while preserving spatial information about the tissue context or subcellular localization of analysed nucleic acids. In this Innovation article, we summarize pioneering technologies that enable spatially resolved transcriptomics and discuss how these methods have the potential to extend beyond transcriptomics to encompass spatially resolved genomics, proteomics and possibly other omic disciplines.

KW - Gene Expression Profiling

KW - High-Throughput Screening Assays

KW - Molecular Imaging

KW - Sequence Analysis

KW - Single-Cell Analysis

KW - Spatial Analysis

U2 - 10.1038/nrg3832

DO - 10.1038/nrg3832

M3 - Article

C2 - 25446315

SN - 1471-0056

VL - 16

SP - 57

EP - 66

JO - Nature Reviews Genetics

JF - Nature Reviews Genetics

IS - 1

ER -