Species of Phaeoacremonium associated with infections in humans and environmental reservoirs in infected woody plants

TY - JOUR

T1 - Species of Phaeoacremonium associated with infections in humans and environmental reservoirs in infected woody plants

AU - Mostert, Lizel

AU - Groenewald, Johannes Z

AU - Summerbell, Richard C

AU - Robert, Vincent

AU - Sutton, Deanna A

AU - Padhye, Arvind A

AU - Crous, Pedro W

PY - 2005/4

Y1 - 2005/4

N2 - To date, three species of Phaeoacremonium have been associated with phaeohyphomycosis. These are P. parasiticum (formerly Phialophora parasitica), P. inflatipes, and P. rubrigenum. Numerous unknown isolates resembling Phaeoacremonium spp. have in recent years been isolated from human patients as well as from woody plants that appear to be the main environmental source of these fungi. Nine new Phaeoacremonium species, of which six were obtained as etiologic agents of human opportunistic infection, are reported. They can be identified based on their cultural and morphological characters, and the identifications are strongly supported in phylogenetic analyses of partial sequences of the actin, beta-tubulin, and calmodulin genes. A multiple-entry electronic key based on morphological, cultural, and beta-tubulin sequence data was developed to facilitate routine species identification. Reexamination of all isolates of P. inflatipes associated with human disease showed them to be misidentified and to belong to the new taxa described here.

AB - To date, three species of Phaeoacremonium have been associated with phaeohyphomycosis. These are P. parasiticum (formerly Phialophora parasitica), P. inflatipes, and P. rubrigenum. Numerous unknown isolates resembling Phaeoacremonium spp. have in recent years been isolated from human patients as well as from woody plants that appear to be the main environmental source of these fungi. Nine new Phaeoacremonium species, of which six were obtained as etiologic agents of human opportunistic infection, are reported. They can be identified based on their cultural and morphological characters, and the identifications are strongly supported in phylogenetic analyses of partial sequences of the actin, beta-tubulin, and calmodulin genes. A multiple-entry electronic key based on morphological, cultural, and beta-tubulin sequence data was developed to facilitate routine species identification. Reexamination of all isolates of P. inflatipes associated with human disease showed them to be misidentified and to belong to the new taxa described here.

KW - Actinidia

KW - Actins

KW - Ascomycota

KW - Calmodulin

KW - DNA, Fungal

KW - Environmental Microbiology

KW - Humans

KW - Molecular Sequence Data

KW - Mycological Typing Techniques

KW - Mycoses

KW - Phenotype

KW - Phylogeny

KW - Plants

KW - Prunus

KW - Sequence Analysis, DNA

KW - Species Specificity

KW - Tubulin

KW - Vitis

U2 - 10.1128/JCM.43.4.1752-1767.2005

DO - 10.1128/JCM.43.4.1752-1767.2005

M3 - Article

C2 - 15814996

SN - 0095-1137

VL - 43

SP - 1752

EP - 1767

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 4

ER -