Stem cells marked by the R-spondin receptor LGR5

TY - JOUR

T1 - Stem cells marked by the R-spondin receptor LGR5

AU - Koo, Bon-Kyoung

AU - Clevers, Hans

N1 - Copyright © 2014 AGA Institute. Published by Elsevier Inc. All rights reserved.

PY - 2014/8

Y1 - 2014/8

N2 - Since the discovery of LGR5 as a marker of intestinal stem cells, the field has developed explosively and led to many new avenues of research. The inner workings of the intestinal crypt stem cell niche are now well understood. The study of stem cell-enriched genes has uncovered some previously unknown aspects of the Wnt signaling pathway, the major driver of crypt dynamics. LGR5(+) stem cells can now be cultured over long periods in vitro as epithelial organoids or "mini-guts." This technology opens new possibilities of using cultured adult stem cells for drug development, disease modeling, gene therapy, and regenerative medicine. This review describes the rediscovery of crypt base columnar cells as LGR5(+) adult stem cells and summarizes subsequent progress, promises, unresolved issues, and challenges of the field.

AB - Since the discovery of LGR5 as a marker of intestinal stem cells, the field has developed explosively and led to many new avenues of research. The inner workings of the intestinal crypt stem cell niche are now well understood. The study of stem cell-enriched genes has uncovered some previously unknown aspects of the Wnt signaling pathway, the major driver of crypt dynamics. LGR5(+) stem cells can now be cultured over long periods in vitro as epithelial organoids or "mini-guts." This technology opens new possibilities of using cultured adult stem cells for drug development, disease modeling, gene therapy, and regenerative medicine. This review describes the rediscovery of crypt base columnar cells as LGR5(+) adult stem cells and summarizes subsequent progress, promises, unresolved issues, and challenges of the field.

KW - Adult Stem Cells

KW - Animals

KW - Biological Markers

KW - Cell Differentiation

KW - Cell Lineage

KW - Cell Proliferation

KW - Cells, Cultured

KW - Gene Expression Regulation

KW - Genetic Engineering

KW - Humans

KW - Intestines

KW - Organoids

KW - Receptors, G-Protein-Coupled

KW - Time Factors

KW - Wnt Signaling Pathway

U2 - 10.1053/j.gastro.2014.05.007

DO - 10.1053/j.gastro.2014.05.007

M3 - Article

C2 - 24859206

SN - 0016-5085

VL - 147

SP - 289

EP - 302

JO - Gastroenterology

JF - Gastroenterology

IS - 2

ER -