Surgical implantation of an abdominal imaging window for intravital microscopy

L. Ritsma; E.J. Steller; S.I. Ellenbroek; O. Kranenburg; I.H. Rinkes; J. van Rheenen

doi:10.1038/nprot.2013.026

Surgical implantation of an abdominal imaging window for intravital microscopy

L. Ritsma, E.J. Steller, S.I. Ellenbroek, O. Kranenburg, I.H. Rinkes, J. van Rheenen

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

210 Citaten (Scopus)

Samenvatting

High-resolution intravital microscopy through imaging windows has become an indispensable technique for the long-term visualization of dynamic processes in living animals. Easily accessible sites such as the skin, the breast and the skull can be imaged using various different imaging windows; however, long-term imaging studies on cellular processes in abdominal organs are more challenging. These processes include colonization of the liver by metastatic tumor cells and the development of an immune response in the spleen. We have recently developed an abdominal imaging window (AIW) that allows long-term imaging of the liver, the pancreas, the intestine, the kidney and the spleen. Here we describe the detailed protocol for the optimal surgical implantation of the AIW, which takes approximately 1 h, and subsequent multiphoton imaging, which takes up to 1 month.

Originele taal-2	Engels
Pagina's (van-tot)	583-594
Tijdschrift	Nature Protocols
Volume	8
Nummer van het tijdschrift	3
DOI's	https://doi.org/10.1038/nprot.2013.026
Status	Gepubliceerd - 2013

Toegang tot document

10.1038/nprot.2013.026

Citeer dit

@article{7b96014d35eb41b9896ef5fbf2795dfa,

title = "Surgical implantation of an abdominal imaging window for intravital microscopy",

abstract = "High-resolution intravital microscopy through imaging windows has become an indispensable technique for the long-term visualization of dynamic processes in living animals. Easily accessible sites such as the skin, the breast and the skull can be imaged using various different imaging windows; however, long-term imaging studies on cellular processes in abdominal organs are more challenging. These processes include colonization of the liver by metastatic tumor cells and the development of an immune response in the spleen. We have recently developed an abdominal imaging window (AIW) that allows long-term imaging of the liver, the pancreas, the intestine, the kidney and the spleen. Here we describe the detailed protocol for the optimal surgical implantation of the AIW, which takes approximately 1 h, and subsequent multiphoton imaging, which takes up to 1 month.",

author = "L. Ritsma and E.J. Steller and S.I. Ellenbroek and O. Kranenburg and I.H. Rinkes and {van Rheenen}, J.",

note = "Reporting year: 2013",

year = "2013",

doi = "10.1038/nprot.2013.026",

language = "English",

volume = "8",

pages = "583--594",

journal = "Nature Protocols",

issn = "1754-2189",

publisher = "Nature Publishing Group",

number = "3",

}

TY - JOUR

T1 - Surgical implantation of an abdominal imaging window for intravital microscopy

AU - Ritsma, L.

AU - Steller, E.J.

AU - Ellenbroek, S.I.

AU - Kranenburg, O.

AU - Rinkes, I.H.

AU - van Rheenen, J.

N1 - Reporting year: 2013

PY - 2013

Y1 - 2013

N2 - High-resolution intravital microscopy through imaging windows has become an indispensable technique for the long-term visualization of dynamic processes in living animals. Easily accessible sites such as the skin, the breast and the skull can be imaged using various different imaging windows; however, long-term imaging studies on cellular processes in abdominal organs are more challenging. These processes include colonization of the liver by metastatic tumor cells and the development of an immune response in the spleen. We have recently developed an abdominal imaging window (AIW) that allows long-term imaging of the liver, the pancreas, the intestine, the kidney and the spleen. Here we describe the detailed protocol for the optimal surgical implantation of the AIW, which takes approximately 1 h, and subsequent multiphoton imaging, which takes up to 1 month.

AB - High-resolution intravital microscopy through imaging windows has become an indispensable technique for the long-term visualization of dynamic processes in living animals. Easily accessible sites such as the skin, the breast and the skull can be imaged using various different imaging windows; however, long-term imaging studies on cellular processes in abdominal organs are more challenging. These processes include colonization of the liver by metastatic tumor cells and the development of an immune response in the spleen. We have recently developed an abdominal imaging window (AIW) that allows long-term imaging of the liver, the pancreas, the intestine, the kidney and the spleen. Here we describe the detailed protocol for the optimal surgical implantation of the AIW, which takes approximately 1 h, and subsequent multiphoton imaging, which takes up to 1 month.

U2 - 10.1038/nprot.2013.026

DO - 10.1038/nprot.2013.026

M3 - Article

SN - 1754-2189

VL - 8

SP - 583

EP - 594

JO - Nature Protocols

JF - Nature Protocols

IS - 3

ER -

Surgical implantation of an abdominal imaging window for intravital microscopy

Samenvatting

Toegang tot document

Vingerafdruk

Citeer dit