Technical advance in fungal biotechnology: development of a miniaturized culture method and an automated high-throughput screening

F Alberto, D Navarro, R P de Vries, M Asther, E Record

    Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

    Samenvatting

    AIMS: The goal of the study was to develop a reliable, reproducible and rapid method of culture in order to screen a large number of fungal transformants.

    METHODS AND RESULTS: The method is based upon miniaturized cell cultures and automated expression screening in microwell plates. For the method development, 50 recombinant Aspergillus vadensis clones producing feruloyl esterase B (FaeB) from Aspergillus niger were screened in 6 days. Then a panel of clones showing various behaviours was checked in flasks in order to demonstrate the reproducibility of the method. Using this method, a transformant of A. vadensis producing 1.2 g l(-1) of FaeB was selected (12-fold more than the A. niger overproducing strain).

    CONCLUSIONS: This miniaturized culture method allows to obtain reliable and reproducible results. The procedure has the advantages of being efficient, time-saving and more efficient than conventional in-flask culture screening as it can screen 800 clones per day after a culture of 3 days.

    SIGNIFICANCE AND IMPACT OF THE STUDY: This method could be applied to any other fungal strain culture, enzyme activity or biodiversity screening.

    Originele taal-2Engels
    Pagina's (van-tot)278-82
    Aantal pagina's5
    TijdschriftLetters in Applied Microbiology
    Volume49
    Nummer van het tijdschrift2
    DOI's
    StatusGepubliceerd - aug 2009

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