Temporally replicated reduced representation bisulfate sequencing data on DNA methylation patterns in great tit.

TY - JOUR

T1 - Temporally replicated reduced representation bisulfate sequencing data on DNA methylation patterns in great tit.

AU - Mäkinen, Hannu

AU - Viitaniemi, Heidi

AU - Visser, M.E.

AU - Verhagen, Irene C.

AU - van Oers, K.

AU - Husby, Arild

N1 - 6792, AnE; Data archiving: Data archived at NCBI and at Figshare: The data described here consists of sequence reads deposited in NCBI Sequence Read Archive37. All the libraries and the individual fastq files contained in them are deposited under accessions SRX3209916-SRX320991937,38,39,40. The Figshare records25 comprise of four files (not NIOO data)

PY - 2019

Y1 - 2019

N2 - Seasonal timing of reproduction is an important fitness trait in many plants and animals but the underlying molecular mechanism for this trait is poorly known. DNA methylation is known to affect timing of reproduction in various organisms and is therefore a potential mechanism also in birds. Here we describe genome wide data aiming to detect temporal changes in methylation in relation to timing of breeding using artificial selection lines of great tits (Parus major) exposed to contrasting temperature treatments. Methylation levels of DNA extracted from erythrocytes were examined using reduced representation bisulfite sequencing (RRBS). In total, we obtained sequencing data from 63 libraries over four different time points from 16 birds with on average 20 million quality filtered reads per library. These data describe individual level temporal variation in DNA methylation throughout the breeding season under experimental temperature regimes and provides a resource for future studies investigating the role of temporal changes in DNA methylation in timing of reproduction.

AB - Seasonal timing of reproduction is an important fitness trait in many plants and animals but the underlying molecular mechanism for this trait is poorly known. DNA methylation is known to affect timing of reproduction in various organisms and is therefore a potential mechanism also in birds. Here we describe genome wide data aiming to detect temporal changes in methylation in relation to timing of breeding using artificial selection lines of great tits (Parus major) exposed to contrasting temperature treatments. Methylation levels of DNA extracted from erythrocytes were examined using reduced representation bisulfite sequencing (RRBS). In total, we obtained sequencing data from 63 libraries over four different time points from 16 birds with on average 20 million quality filtered reads per library. These data describe individual level temporal variation in DNA methylation throughout the breeding season under experimental temperature regimes and provides a resource for future studies investigating the role of temporal changes in DNA methylation in timing of reproduction.

KW - international

U2 - 10.1038/s41597-019-0136-0

DO - 10.1038/s41597-019-0136-0

M3 - Article

SN - 2052-4463

VL - 6

JO - Scientific data

JF - Scientific data

M1 - 136

ER -