The C2H2 transcription factor SltA is required for germination and hyphal development in Aspergillus fumigatus

TY - JOUR

T1 - The C2H2 transcription factor SltA is required for germination and hyphal development in Aspergillus fumigatus

AU - Baltussen, Tim J.H.

AU - van Rhijn, Norman

AU - Coolen, Jordy P.M.

AU - Dijksterhuis, Jan

AU - Verweij, Paul E.

AU - Bromley, Michael J.

AU - Melchers, Willem J.G.

N1 - Publisher Copyright: © 2023 Baltussen et al.

PY - 2023/8

Y1 - 2023/8

N2 - ABSTRACT Germination of inhaled Aspergillus fumigatus conidia is a necessary sequitur for infection. Germination of conidia starts with the breaking of dormancy, which is initiated by an increase of the cellular perimeter in a process termed isotropic growth. This swelling phase is followed by polarized growth, resulting in the formation of a germ tube. The multinucleate tubular cells exhibit tip growth from the hyphae, after which lateral branches emerge to form the mycelial network. The regulatory mechanisms governing conidial germination are not well defined.In this study, we identifieda novel role for the transcription factor SltA in the orchestration of germination and hyphal development. Conidia lacking sltA fail to appropriately regulate isotropic growth and begin to swell earlier and subsequently switch to polarized growth faster. Additionally, hyphal development is distorted in a ΔsltA isolate as hyphae are hyper-branching and wider, and show branching at the apical tip. ΔsltA conidia are more tolerant to cell wall stressors on minimal medium compared to the wild-type (WT) strain. A transcriptome analysis of differentstages of early growth was carried out to assess the regulatory role of SltA. Null mutants generated for three of the most dysregulated genes showed rapid germ tube emergence. Distinct from the phenotype observed for ΔsltA, conidia from these strains lacked defects in isotropic growth, but switched to polarized growth faster. Here, we characterize and describe several genes in the regulon of SltA, highlighting the complex nature of germination.

AB - ABSTRACT Germination of inhaled Aspergillus fumigatus conidia is a necessary sequitur for infection. Germination of conidia starts with the breaking of dormancy, which is initiated by an increase of the cellular perimeter in a process termed isotropic growth. This swelling phase is followed by polarized growth, resulting in the formation of a germ tube. The multinucleate tubular cells exhibit tip growth from the hyphae, after which lateral branches emerge to form the mycelial network. The regulatory mechanisms governing conidial germination are not well defined.In this study, we identifieda novel role for the transcription factor SltA in the orchestration of germination and hyphal development. Conidia lacking sltA fail to appropriately regulate isotropic growth and begin to swell earlier and subsequently switch to polarized growth faster. Additionally, hyphal development is distorted in a ΔsltA isolate as hyphae are hyper-branching and wider, and show branching at the apical tip. ΔsltA conidia are more tolerant to cell wall stressors on minimal medium compared to the wild-type (WT) strain. A transcriptome analysis of differentstages of early growth was carried out to assess the regulatory role of SltA. Null mutants generated for three of the most dysregulated genes showed rapid germ tube emergence. Distinct from the phenotype observed for ΔsltA, conidia from these strains lacked defects in isotropic growth, but switched to polarized growth faster. Here, we characterize and describe several genes in the regulon of SltA, highlighting the complex nature of germination.

KW - Aspergillus fumigatus

KW - germination

KW - hyphal development

KW - SltA

KW - transcriptional regulation

UR - http://www.scopus.com/inward/record.url?scp=85168799641&partnerID=8YFLogxK

U2 - 10.1128/msphere.00076-23

DO - 10.1128/msphere.00076-23

M3 - Article

C2 - 37260230

AN - SCOPUS:85168799641

SN - 2379-5042

VL - 8

JO - mSphere

JF - mSphere

IS - 4

ER -