The Hypocrea jecorina (syn. Trichoderma reesei) lxr1 gene encodes a D-mannitol dehydrogenase and is not involved in L-arabinose catabolism

Benjamin Metz, Ronald P de Vries, Stefan Polak, Verena Seidl, Bernhard Seiboth

    Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

    Samenvatting

    The Hypocrea jecorina LXR1 was described as the first fungal L-xylulose reductase responsible for NADPH dependent reduction of L-xylulose to xylitol in L-arabinose catabolism. Phylogenetic analysis now reveals that LXR1 forms a clade with fungal D-mannitol 2-dehydrogenases. Lxr1 and the orthologous Aspergillus niger mtdA are not induced by L-arabinose but expressed at low levels during growth on different carbon sources. Deletion of lxr1 does not affect growth on L-arabinose and L-xylulose reductase activity remains unaltered whereas D-mannitol 2-dehydrogenase activities are reduced. We conclude that LXR1 is a D-mannitol 2-dehydrogenase and that a true LXR1 is still awaiting discovery.

    Originele taal-2Engels
    Pagina's (van-tot)1309-13
    Aantal pagina's5
    TijdschriftFEBS Letters
    Volume583
    Nummer van het tijdschrift8
    DOI's
    StatusGepubliceerd - 17 apr 2009

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