The microRNA-producing enzyme Dicer1 is essential for zebrafish development

TY - JOUR

T1 - The microRNA-producing enzyme Dicer1 is essential for zebrafish development

AU - Wienholds, E.

AU - Koudijs, M.J.

AU - van Eeden, F.

AU - Cuppen, E.

AU - Plasterk, R.

N1 - Reporting year: 2003 Metis note: 14528306

PY - 2003

Y1 - 2003

N2 - MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.

AB - MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.

U2 - 10.1038/ng1251

DO - 10.1038/ng1251

M3 - Article

SN - 1061-4036

VL - 35

SP - 217

EP - 218

JO - Nature Genetics

JF - Nature Genetics

IS - 3

ER -