The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors

Xiaoyi Chen; Juan P. Moran Torres; Peter Jan Vonk; J. Mirjam A. Damen; Karli R. Reiding; Jan Dijksterhuis; Luis G. Lugones; Han A.B. Wösten

doi:10.1016/j.fgb.2024.103894

The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors

Xiaoyi Chen, Juan P. Moran Torres, Peter Jan Vonk, J. Mirjam A. Damen, Karli R. Reiding, Jan Dijksterhuis, Luis G. Lugones, Han A.B. Wösten^*

^*Bijbehorende auteur voor dit werk

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

2 Citaten (Scopus)

Samenvatting

Inactivation of flbA in Aspergillus niger results in thinner cell walls, increased cell lysis, abolished sporulation, and an increased secretome complexity. A total of 36 transcription factor (TF) genes are differentially expressed in ΔflbA. Here, seven of these genes (abaA, aslA, aslB, azf1, htfA, nosA, and srbA) were inactivated. Inactivation of each of these genes affected sporulation and, with the exception of abaA, cell wall integrity and protein secretion. The impact on secretion was strongest in the case of ΔaslA and ΔaslB that showed increased pepsin, cellulase, and amylase activity. Biomass was reduced of agar cultures of ΔabaA, ΔaslA, ΔnosA, and ΔsrbA, while biomass was higher in liquid shaken cultures of ΔaslA and ΔaslB. The ΔaslA and ΔhtfA strains showed increased resistance to H₂O₂, while ΔaslB was more sensitive to this reactive oxygen species. Together, inactivation of the seven TF genes impacted biomass formation, sporulation, protein secretion, and stress resistance, and thereby these genes explain at least part of the pleiotropic phenotype of ΔflbA of A. niger.

Originele taal-2	Engels
Artikelnummer	103894
Tijdschrift	Fungal Genetics and Biology
Volume	172
DOI's	https://doi.org/10.1016/j.fgb.2024.103894
Status	Gepubliceerd - jun. 2024

Toegang tot document

10.1016/j.fgb.2024.103894

Andere bestanden en links

Link naar publicatie in Scopus

Citeer dit

Chen, X., Moran Torres, J. P., Jan Vonk, P., Damen, J. M. A., Reiding, K. R., Dijksterhuis, J., Lugones, L. G., & Wösten, H. A. B. (2024). The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors. Fungal Genetics and Biology, 172, Artikel 103894. https://doi.org/10.1016/j.fgb.2024.103894

@article{243202a029f2485aa555c46b95770855,

title = "The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors",

abstract = "Inactivation of flbA in Aspergillus niger results in thinner cell walls, increased cell lysis, abolished sporulation, and an increased secretome complexity. A total of 36 transcription factor (TF) genes are differentially expressed in ΔflbA. Here, seven of these genes (abaA, aslA, aslB, azf1, htfA, nosA, and srbA) were inactivated. Inactivation of each of these genes affected sporulation and, with the exception of abaA, cell wall integrity and protein secretion. The impact on secretion was strongest in the case of ΔaslA and ΔaslB that showed increased pepsin, cellulase, and amylase activity. Biomass was reduced of agar cultures of ΔabaA, ΔaslA, ΔnosA, and ΔsrbA, while biomass was higher in liquid shaken cultures of ΔaslA and ΔaslB. The ΔaslA and ΔhtfA strains showed increased resistance to H2O2, while ΔaslB was more sensitive to this reactive oxygen species. Together, inactivation of the seven TF genes impacted biomass formation, sporulation, protein secretion, and stress resistance, and thereby these genes explain at least part of the pleiotropic phenotype of ΔflbA of A. niger.",

keywords = "Asexual development, Aspergillus, Cell wall stress, FlbA, Fungus, Secretion",

author = "Xiaoyi Chen and {Moran Torres}, {Juan P.} and {Jan Vonk}, Peter and Damen, {J. Mirjam A.} and Reiding, {Karli R.} and Jan Dijksterhuis and Lugones, {Luis G.} and W{\"o}sten, {Han A.B.}",

note = "Publisher Copyright: {\textcopyright} 2024 The Author(s)",

year = "2024",

month = jun,

doi = "10.1016/j.fgb.2024.103894",

language = "English",

volume = "172",

journal = "Fungal Genetics and Biology",

issn = "1087-1845",

publisher = "Academic Press",

}

The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors. / Chen, Xiaoyi; Moran Torres, Juan P.; Jan Vonk, Peter et al.
In: Fungal Genetics and Biology, Vol. 172, 103894, 06.2024.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

TY - JOUR

T1 - The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors

AU - Chen, Xiaoyi

AU - Moran Torres, Juan P.

AU - Jan Vonk, Peter

AU - Damen, J. Mirjam A.

AU - Reiding, Karli R.

AU - Dijksterhuis, Jan

AU - Lugones, Luis G.

AU - Wösten, Han A.B.

PY - 2024/6

Y1 - 2024/6

N2 - Inactivation of flbA in Aspergillus niger results in thinner cell walls, increased cell lysis, abolished sporulation, and an increased secretome complexity. A total of 36 transcription factor (TF) genes are differentially expressed in ΔflbA. Here, seven of these genes (abaA, aslA, aslB, azf1, htfA, nosA, and srbA) were inactivated. Inactivation of each of these genes affected sporulation and, with the exception of abaA, cell wall integrity and protein secretion. The impact on secretion was strongest in the case of ΔaslA and ΔaslB that showed increased pepsin, cellulase, and amylase activity. Biomass was reduced of agar cultures of ΔabaA, ΔaslA, ΔnosA, and ΔsrbA, while biomass was higher in liquid shaken cultures of ΔaslA and ΔaslB. The ΔaslA and ΔhtfA strains showed increased resistance to H2O2, while ΔaslB was more sensitive to this reactive oxygen species. Together, inactivation of the seven TF genes impacted biomass formation, sporulation, protein secretion, and stress resistance, and thereby these genes explain at least part of the pleiotropic phenotype of ΔflbA of A. niger.

AB - Inactivation of flbA in Aspergillus niger results in thinner cell walls, increased cell lysis, abolished sporulation, and an increased secretome complexity. A total of 36 transcription factor (TF) genes are differentially expressed in ΔflbA. Here, seven of these genes (abaA, aslA, aslB, azf1, htfA, nosA, and srbA) were inactivated. Inactivation of each of these genes affected sporulation and, with the exception of abaA, cell wall integrity and protein secretion. The impact on secretion was strongest in the case of ΔaslA and ΔaslB that showed increased pepsin, cellulase, and amylase activity. Biomass was reduced of agar cultures of ΔabaA, ΔaslA, ΔnosA, and ΔsrbA, while biomass was higher in liquid shaken cultures of ΔaslA and ΔaslB. The ΔaslA and ΔhtfA strains showed increased resistance to H2O2, while ΔaslB was more sensitive to this reactive oxygen species. Together, inactivation of the seven TF genes impacted biomass formation, sporulation, protein secretion, and stress resistance, and thereby these genes explain at least part of the pleiotropic phenotype of ΔflbA of A. niger.

KW - Asexual development

KW - Aspergillus

KW - Cell wall stress

KW - FlbA

KW - Fungus

KW - Secretion

UR - http://www.scopus.com/inward/record.url?scp=85191022175&partnerID=8YFLogxK

U2 - 10.1016/j.fgb.2024.103894

DO - 10.1016/j.fgb.2024.103894

M3 - Article

C2 - 38657897

AN - SCOPUS:85191022175

SN - 1087-1845

VL - 172

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

M1 - 103894

ER -