Tissue- and expression level-specific chromatin looping at maize b1 epialleles.

M. Louwers; R. Bader; M. Haring; R. van Driel; W. de Laat; M. Stam

doi:10.1105/tpc.108.064329

Tissue- and expression level-specific chromatin looping at maize b1 epialleles.

M. Louwers, R. Bader, M. Haring, R. van Driel, W. de Laat, M. Stam

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

103 Citaten (Scopus)

Samenvatting

This work examines the involvement of chromatin looping in the transcriptional regulation of two epialleles of the maize (Zea mays) b1 gene, B-I and B'. These two epialleles are tissue-specifically regulated and are involved in paramutation. B-I and B' are expressed at high and low levels, respectively. A hepta-repeat approximately 100 kb upstream of the transcription start site (TSS) is required for both paramutation and high b1 expression. Using chromosome conformation capture, we show that the hepta-repeat physically interacts with the TSS region in a tissue- and expression level-specific manner. Multiple repeats are required to stabilize this interaction. High b1 expression is mediated by a multiloop structure; besides the hepta-repeat, other sequence regions physically interact with the TSS as well, and these interactions are epiallele- and expression level-specific. Formaldehyde-assisted isolation of regulatory elements uncovered multiple interacting regions as potentially regulatory.

Originele taal-2	Engels
Pagina's (van-tot)	832-842
Tijdschrift	Plant Cell
Volume	21
Nummer van het tijdschrift	3
DOI's	https://doi.org/10.1105/tpc.108.064329
Status	Gepubliceerd - 2009

Toegang tot document

10.1105/tpc.108.064329

Citeer dit

@article{52f129ef73d84fbe8aafc218557a97bc,

title = "Tissue- and expression level-specific chromatin looping at maize b1 epialleles.",

abstract = "This work examines the involvement of chromatin looping in the transcriptional regulation of two epialleles of the maize (Zea mays) b1 gene, B-I and B'. These two epialleles are tissue-specifically regulated and are involved in paramutation. B-I and B' are expressed at high and low levels, respectively. A hepta-repeat approximately 100 kb upstream of the transcription start site (TSS) is required for both paramutation and high b1 expression. Using chromosome conformation capture, we show that the hepta-repeat physically interacts with the TSS region in a tissue- and expression level-specific manner. Multiple repeats are required to stabilize this interaction. High b1 expression is mediated by a multiloop structure; besides the hepta-repeat, other sequence regions physically interact with the TSS as well, and these interactions are epiallele- and expression level-specific. Formaldehyde-assisted isolation of regulatory elements uncovered multiple interacting regions as potentially regulatory.",

author = "M. Louwers and R. Bader and M. Haring and {van Driel}, R. and {de Laat}, W. and M. Stam",

note = "Reporting year: 2009",

year = "2009",

doi = "10.1105/tpc.108.064329",

language = "English",

volume = "21",

pages = "832--842",

journal = "Plant Cell",

issn = "1040-4651",

publisher = "American Society of Plant Biologists",

number = "3",

}

TY - JOUR

T1 - Tissue- and expression level-specific chromatin looping at maize b1 epialleles.

AU - Louwers, M.

AU - Bader, R.

AU - Haring, M.

AU - van Driel, R.

AU - de Laat, W.

AU - Stam, M.

N1 - Reporting year: 2009

PY - 2009

Y1 - 2009

N2 - This work examines the involvement of chromatin looping in the transcriptional regulation of two epialleles of the maize (Zea mays) b1 gene, B-I and B'. These two epialleles are tissue-specifically regulated and are involved in paramutation. B-I and B' are expressed at high and low levels, respectively. A hepta-repeat approximately 100 kb upstream of the transcription start site (TSS) is required for both paramutation and high b1 expression. Using chromosome conformation capture, we show that the hepta-repeat physically interacts with the TSS region in a tissue- and expression level-specific manner. Multiple repeats are required to stabilize this interaction. High b1 expression is mediated by a multiloop structure; besides the hepta-repeat, other sequence regions physically interact with the TSS as well, and these interactions are epiallele- and expression level-specific. Formaldehyde-assisted isolation of regulatory elements uncovered multiple interacting regions as potentially regulatory.

AB - This work examines the involvement of chromatin looping in the transcriptional regulation of two epialleles of the maize (Zea mays) b1 gene, B-I and B'. These two epialleles are tissue-specifically regulated and are involved in paramutation. B-I and B' are expressed at high and low levels, respectively. A hepta-repeat approximately 100 kb upstream of the transcription start site (TSS) is required for both paramutation and high b1 expression. Using chromosome conformation capture, we show that the hepta-repeat physically interacts with the TSS region in a tissue- and expression level-specific manner. Multiple repeats are required to stabilize this interaction. High b1 expression is mediated by a multiloop structure; besides the hepta-repeat, other sequence regions physically interact with the TSS as well, and these interactions are epiallele- and expression level-specific. Formaldehyde-assisted isolation of regulatory elements uncovered multiple interacting regions as potentially regulatory.

U2 - 10.1105/tpc.108.064329

DO - 10.1105/tpc.108.064329

M3 - Article

SN - 1040-4651

VL - 21

SP - 832

EP - 842

JO - Plant Cell

JF - Plant Cell

IS - 3

ER -

Tissue- and expression level-specific chromatin looping at maize b1 epialleles.

Samenvatting

Toegang tot document

Vingerafdruk

Citeer dit