Tissue- and expression level-specific chromatin looping at maize b1 epialleles.

M. Louwers, R. Bader, M. Haring, R. van Driel, W. de Laat, M. Stam

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

This work examines the involvement of chromatin looping in the transcriptional regulation of two epialleles of the maize (Zea mays) b1 gene, B-I and B'. These two epialleles are tissue-specifically regulated and are involved in paramutation. B-I and B' are expressed at high and low levels, respectively. A hepta-repeat approximately 100 kb upstream of the transcription start site (TSS) is required for both paramutation and high b1 expression. Using chromosome conformation capture, we show that the hepta-repeat physically interacts with the TSS region in a tissue- and expression level-specific manner. Multiple repeats are required to stabilize this interaction. High b1 expression is mediated by a multiloop structure; besides the hepta-repeat, other sequence regions physically interact with the TSS as well, and these interactions are epiallele- and expression level-specific. Formaldehyde-assisted isolation of regulatory elements uncovered multiple interacting regions as potentially regulatory.
Originele taal-2Engels
Pagina's (van-tot)832-842
TijdschriftPlant Cell
Volume21
Nummer van het tijdschrift3
DOI's
StatusGepubliceerd - 2009

Vingerafdruk

Duik in de onderzoeksthema's van 'Tissue- and expression level-specific chromatin looping at maize b1 epialleles.'. Samen vormen ze een unieke vingerafdruk.

Citeer dit