@article{59fa3ea20d2b4cb683de76257c808a01,
title = "Transcriptional profiling of cells sorted by RNA abundance",
abstract = "We have developed a quantitative technique for sorting cells on the basis of endogenous RNA abundance, with a molecular resolution of 10-20 transcripts. We demonstrate efficient and unbiased RNA extraction from transcriptionally sorted cells and report a high-fidelity transcriptome measurement of mouse induced pluripotent stem cells (iPSCs) isolated from a heterogeneous reprogramming culture. This method is broadly applicable to profiling transcriptionally distinct cellular states without requiring antibodies or transgenic fluorescent proteins.",
keywords = "Alleles, Animals, Cell Culture Techniques, Cellular Reprogramming, Doxycycline, Embryonic Stem Cells, Fibroblasts, Flow Cytometry, Gene Expression Profiling, Genome-Wide Association Study, Green Fluorescent Proteins, In Situ Hybridization, Fluorescence, Induced Pluripotent Stem Cells, Mice, NIH 3T3 Cells, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, RNA, RNA, Messenger, Transcription, Genetic, Transgenes",
author = "Sandy Klemm and Stefan Semrau and Kay Wiebrands and Dylan Mooijman and Faddah, {Dina A} and Rudolf Jaenisch and {van Oudenaarden}, Alexander",
year = "2014",
month = may,
doi = "10.1038/nmeth.2910",
language = "English",
volume = "11",
pages = "549--51",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "5",
}