Transcriptome and proteome analyses of proteases in biotroph fungal pathogen Cladosporium fulvum

TY - JOUR

T1 - Transcriptome and proteome analyses of proteases in biotroph fungal pathogen Cladosporium fulvum

AU - Karimi Jashni, Mansoor

AU - van der Burgt, Ate

AU - Battaglia, Evy

AU - Mehrabi, Rahim

AU - Collemare, Jérôme

AU - de Wit, Pierre J.G.M.

N1 - Funding Information: We are grateful to financial assistance for this research provided by Wageningen University and the Ministry of Science, Research and Technology (MSRT) of Iran. We do thank Dr. S. Boren in department of Biochemistry-Wageningen University for performing mass spectrometry analysis. Funding Information: We are grateful to financial assistance for this research provided by Wageningen University and the Ministry of Science, Research and Technology (MSRT) of Iran. We do thank Dr. S. Boren in department of Biochemistry-Wageningen University for performing mass spectrometry analysis. Publisher Copyright: © 2019, Società Italiana di Patologia Vegetale (S.I.Pa.V.).

PY - 2020/5/1

Y1 - 2020/5/1

N2 - Proteases are key components of the hydrolytic enzyme arsenal employed by fungal pathogens to invade their host plants. The recent advances in -omics era have facilitated identification of functional proteases involved in plant-fungus interactions. By comparison of the publically available sequences of fungal genomes we found that the number of protease genes present in the genome of Cladosporium fulvum, a biotrophic tomato pathogen, is comparable with that of hemibiotrophs. To identify host plant inducible protease genes and their products, we performed transcriptome and proteome analyses of C. fulvumin vitro and in planta by means of RNA-Seq/RT-qPCR and mass spectrometry. Transcriptome data showed that 14 out of the 59 predicted proteases are expressed during in vitro and in planta growth of C. fulvum, of which nine belong to serine proteases S8 and S10 and the rest belong to metallo- and aspartic proteases. Mass spectrometry confirmed the presence of six proteases at proteome level during plant infection. Expression of limited number of proteases by C. fulvum might sustain biotrophic growth and benefits its stealth pathogenesis.

AB - Proteases are key components of the hydrolytic enzyme arsenal employed by fungal pathogens to invade their host plants. The recent advances in -omics era have facilitated identification of functional proteases involved in plant-fungus interactions. By comparison of the publically available sequences of fungal genomes we found that the number of protease genes present in the genome of Cladosporium fulvum, a biotrophic tomato pathogen, is comparable with that of hemibiotrophs. To identify host plant inducible protease genes and their products, we performed transcriptome and proteome analyses of C. fulvumin vitro and in planta by means of RNA-Seq/RT-qPCR and mass spectrometry. Transcriptome data showed that 14 out of the 59 predicted proteases are expressed during in vitro and in planta growth of C. fulvum, of which nine belong to serine proteases S8 and S10 and the rest belong to metallo- and aspartic proteases. Mass spectrometry confirmed the presence of six proteases at proteome level during plant infection. Expression of limited number of proteases by C. fulvum might sustain biotrophic growth and benefits its stealth pathogenesis.

KW - Cladosoprium fulvum

KW - Fungal proteases

KW - Gene expression

KW - Plant-microbe interaction

KW - Tomato pathogen

UR - http://www.scopus.com/inward/record.url?scp=85074819950&partnerID=8YFLogxK

U2 - 10.1007/s42161-019-00433-0

DO - 10.1007/s42161-019-00433-0

M3 - Article

AN - SCOPUS:85074819950

SN - 1125-4653

VL - 102

SP - 377

EP - 386

JO - Journal of Plant Pathology

JF - Journal of Plant Pathology

IS - 2

ER -