Troy/Tnfrsf19 marks epidermal cells that govern interfollicular epidermal renewal and cornification

Kai Kretzschmar; Kim E Boonekamp; Margit Bleijs; Priyanca Asra; Mandy Koomen; Susana M Chuva de Sousa Lopes; Barbara Giovannone; Hans Clevers

doi:10.1016/j.stemcr.2021.07.007

Troy/Tnfrsf19 marks epidermal cells that govern interfollicular epidermal renewal and cornification

Kai Kretzschmar, Kim E Boonekamp, Margit Bleijs, Priyanca Asra, Mandy Koomen, Susana M Chuva de Sousa Lopes, Barbara Giovannone, Hans Clevers

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

3 Citaten (Scopus)

Samenvatting

The skin epidermis is a highly compartmentalized tissue consisting of a cornifying epithelium called the interfollicular epidermis (IFE) and associated hair follicles (HFs). Several stem cell populations have been described that mark specific compartments in the skin but none of them is specific to the IFE. Here, we identify Troy as a marker of IFE and HF infundibulum basal layer cells in developing and adult human and mouse epidermis. Genetic lineage-tracing experiments demonstrate that Troy-expressing basal cells contribute to long-term renewal of all layers of the cornifying epithelium. Single-cell transcriptomics and organoid assays of Troy-expressing cells, as well as their progeny, confirmed stem cell identity as well as the ability to generate differentiating daughter cells. In conclusion, we define Troy as a marker of epidermal basal cells that govern interfollicular epidermal renewal and cornification.

Originele taal-2	Engels
Pagina's (van-tot)	2379-2394
Aantal pagina's	16
Tijdschrift	Stem Cell Reports
Volume	16
Nummer van het tijdschrift	9
DOI's	https://doi.org/10.1016/j.stemcr.2021.07.007
Status	Gepubliceerd - 14 sep. 2021

Toegang tot document

10.1016/j.stemcr.2021.07.007

Citeer dit

@article{0d92d4d7f7f14ddba3a5aaa632c22917,

title = "Troy/Tnfrsf19 marks epidermal cells that govern interfollicular epidermal renewal and cornification",

abstract = "The skin epidermis is a highly compartmentalized tissue consisting of a cornifying epithelium called the interfollicular epidermis (IFE) and associated hair follicles (HFs). Several stem cell populations have been described that mark specific compartments in the skin but none of them is specific to the IFE. Here, we identify Troy as a marker of IFE and HF infundibulum basal layer cells in developing and adult human and mouse epidermis. Genetic lineage-tracing experiments demonstrate that Troy-expressing basal cells contribute to long-term renewal of all layers of the cornifying epithelium. Single-cell transcriptomics and organoid assays of Troy-expressing cells, as well as their progeny, confirmed stem cell identity as well as the ability to generate differentiating daughter cells. In conclusion, we define Troy as a marker of epidermal basal cells that govern interfollicular epidermal renewal and cornification.",

author = "Kai Kretzschmar and Boonekamp, {Kim E} and Margit Bleijs and Priyanca Asra and Mandy Koomen and {Chuva de Sousa Lopes}, {Susana M} and Barbara Giovannone and Hans Clevers",

year = "2021",

month = sep,

day = "14",

doi = "10.1016/j.stemcr.2021.07.007",

language = "English",

volume = "16",

pages = "2379--2394",

journal = "Stem Cell Reports",

issn = "2213-6711",

publisher = "Cell Press",

number = "9",

}

TY - JOUR

T1 - Troy/Tnfrsf19 marks epidermal cells that govern interfollicular epidermal renewal and cornification

AU - Kretzschmar, Kai

AU - Boonekamp, Kim E

AU - Bleijs, Margit

AU - Asra, Priyanca

AU - Koomen, Mandy

AU - Chuva de Sousa Lopes, Susana M

AU - Giovannone, Barbara

AU - Clevers, Hans

PY - 2021/9/14

Y1 - 2021/9/14

N2 - The skin epidermis is a highly compartmentalized tissue consisting of a cornifying epithelium called the interfollicular epidermis (IFE) and associated hair follicles (HFs). Several stem cell populations have been described that mark specific compartments in the skin but none of them is specific to the IFE. Here, we identify Troy as a marker of IFE and HF infundibulum basal layer cells in developing and adult human and mouse epidermis. Genetic lineage-tracing experiments demonstrate that Troy-expressing basal cells contribute to long-term renewal of all layers of the cornifying epithelium. Single-cell transcriptomics and organoid assays of Troy-expressing cells, as well as their progeny, confirmed stem cell identity as well as the ability to generate differentiating daughter cells. In conclusion, we define Troy as a marker of epidermal basal cells that govern interfollicular epidermal renewal and cornification.

AB - The skin epidermis is a highly compartmentalized tissue consisting of a cornifying epithelium called the interfollicular epidermis (IFE) and associated hair follicles (HFs). Several stem cell populations have been described that mark specific compartments in the skin but none of them is specific to the IFE. Here, we identify Troy as a marker of IFE and HF infundibulum basal layer cells in developing and adult human and mouse epidermis. Genetic lineage-tracing experiments demonstrate that Troy-expressing basal cells contribute to long-term renewal of all layers of the cornifying epithelium. Single-cell transcriptomics and organoid assays of Troy-expressing cells, as well as their progeny, confirmed stem cell identity as well as the ability to generate differentiating daughter cells. In conclusion, we define Troy as a marker of epidermal basal cells that govern interfollicular epidermal renewal and cornification.

U2 - 10.1016/j.stemcr.2021.07.007

DO - 10.1016/j.stemcr.2021.07.007

M3 - Article

C2 - 34358453

SN - 2213-6711

VL - 16

SP - 2379

EP - 2394

JO - Stem Cell Reports

JF - Stem Cell Reports

IS - 9

ER -

Troy/Tnfrsf19 marks epidermal cells that govern interfollicular epidermal renewal and cornification

Samenvatting

Toegang tot document

Vingerafdruk

Citeer dit