TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells

Bin Wu, François Gc Blot, Aaron Benson Wong, Catarina Osório, Youri Adolfs, R Jeroen Pasterkamp, Jana Hartmann, Esther Be Becker, Henk-Jan Boele, Chris I De Zeeuw, Martijn Schonewille

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Despite the canonical homogeneous character of its organization, the cerebellum plays differential computational roles in distinct sensorimotor behaviors. Previously, we showed that Purkinje cell (PC) activity differs between zebrin-negative (Z-) and zebrin-positive (Z+) modules (Zhou et al., 2014). Here, using gain-of-function and loss-of-function mouse models, we show that transient receptor potential cation channel C3 (TRPC3) controls the simple spike activity of Z-, but not Z+ PCs. In addition, TRPC3 regulates complex spike rate and their interaction with simple spikes, exclusively in Z- PCs. At the behavioral level, TRPC3 loss-of-function mice show impaired eyeblink conditioning, which is related to Z- modules, whereas compensatory eye movement adaptation, linked to Z+ modules, is intact. Together, our results indicate that TRPC3 is a major contributor to the cellular heterogeneity that introduces distinct physiological properties in PCs, conjuring functional heterogeneity in cerebellar sensorimotor integration.

Originele taal-2Engels
TijdschrifteLife
Volume8
DOI's
StatusGepubliceerd - 25 mei 2019

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