Unique Phylogenetic Distributions of the Ska and Dam1 Complexes Support Functional Analogy and Suggest Multiple Parallel Displacements of Ska by Dam1

TY - JOUR

T1 - Unique Phylogenetic Distributions of the Ska and Dam1 Complexes Support Functional Analogy and Suggest Multiple Parallel Displacements of Ska by Dam1

AU - van Hooff, Jolien J E

AU - Snel, Berend

AU - Kops, Geert J P L

N1 - © The Author 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

PY - 2017/5/1

Y1 - 2017/5/1

N2 - Faithful chromosome segregation relies on kinetochores, the large protein complexes that connect chromatin to spindle microtubules. Although human and yeast kinetochores are largely homologous, they track microtubules with the unrelated protein complexes Ska (Ska-C, human) and Dam1 (Dam1-C, yeast). We here uncovered that Ska-C and Dam1-C are both widespread among eukaryotes, but in an exceptionally inverse manner, supporting their functional analogy. Within the complexes, all Ska-C and various Dam1-C subunits are ancient paralogs, showing that gene duplication shaped these complexes. We examined various evolutionary scenarios to explain the nearly mutually exclusive patterns of Ska-C and Dam1-C in present-day species. We propose that Ska-C was present in the last eukaryotic common ancestor, that subsequently Dam1-C displaced Ska-C in an early fungus and was horizontally transferred to diverse non-fungal lineages, displacing Ska-C in these lineages too.

AB - Faithful chromosome segregation relies on kinetochores, the large protein complexes that connect chromatin to spindle microtubules. Although human and yeast kinetochores are largely homologous, they track microtubules with the unrelated protein complexes Ska (Ska-C, human) and Dam1 (Dam1-C, yeast). We here uncovered that Ska-C and Dam1-C are both widespread among eukaryotes, but in an exceptionally inverse manner, supporting their functional analogy. Within the complexes, all Ska-C and various Dam1-C subunits are ancient paralogs, showing that gene duplication shaped these complexes. We examined various evolutionary scenarios to explain the nearly mutually exclusive patterns of Ska-C and Dam1-C in present-day species. We propose that Ska-C was present in the last eukaryotic common ancestor, that subsequently Dam1-C displaced Ska-C in an early fungus and was horizontally transferred to diverse non-fungal lineages, displacing Ska-C in these lineages too.

KW - Chromosomal Proteins, Non-Histone

KW - Evolution, Molecular

KW - Gene Duplication

KW - Gene Transfer, Horizontal

KW - Humans

KW - Kinetochores

KW - Microtubule-Associated Proteins

KW - Microtubules

KW - Phylogeny

KW - Saccharomyces cerevisiae

KW - Journal Article

U2 - 10.1093/gbe/evx088

DO - 10.1093/gbe/evx088

M3 - Article

C2 - 28472331

SN - 1759-6653

VL - 9

SP - 1295

EP - 1303

JO - Genome Biology and Evolution

JF - Genome Biology and Evolution

IS - 5

ER -