Using eggshell membranes as a DNA source for population genetic research

TY - JOUR

T1 - Using eggshell membranes as a DNA source for population genetic research

AU - Trimbos, K. B.

AU - Broekman, J.

AU - Kentie, R.

AU - Musters, C. J. M.

AU - de Snoo, G. R.

N1 - ISI Document Delivery No.: 500FI Times Cited: 12 Cited Reference Count: 24 Trimbos, Krijn Baptist Broekman, Joyce Kentie, Rosemarie Musters, Cees J. M. de Snoo, Geert R. de Snoo, geert/M-4315-2013; Musters, Cornelis/K-7828-2013; Kentie, Rosemarie/Z-4600-2019 Kentie, Rosemarie/0000-0001-7050-9265 13 0 10 Springer New york <Go to ISI>://WOS:000270283700021

PY - 2009

Y1 - 2009

N2 - In the context of population genetic research, a faster and less invasive method of DNA sampling would allow large-scale assessments of genetic diversity and genetic differentiation with the help of volunteer observers. The aim of this study was to investigate the usefulness of eggshell membranes as a DNA source for population genetic research, by addressing eggshell membrane DNA quality, degeneration and cross-contamination. To this end, a comparison was made with blood-derived DNA samples. We have demonstrated 100% successful DNA extraction from post-hatched Black-tailed Godwit (Limosa limosa) eggshell membranes as well as from blood samples. Using 11 microsatellite loci, DNA amplification success was 99.1% for eggshell membranes and 97.7% for blood samples. Genetic information within eggshell membrane DNA in comparison to blood DNA was not affected (F(ST) = -0.01735, P = 0.999) by degeneration or possible cross-contamination. Furthermore, neither degeneration nor cross-contamination was apparent in total genotypic comparison of eggshell membrane DNA and blood sample DNA. Our research clearly illustrates that eggshell membranes can be used for population genetic research.

AB - In the context of population genetic research, a faster and less invasive method of DNA sampling would allow large-scale assessments of genetic diversity and genetic differentiation with the help of volunteer observers. The aim of this study was to investigate the usefulness of eggshell membranes as a DNA source for population genetic research, by addressing eggshell membrane DNA quality, degeneration and cross-contamination. To this end, a comparison was made with blood-derived DNA samples. We have demonstrated 100% successful DNA extraction from post-hatched Black-tailed Godwit (Limosa limosa) eggshell membranes as well as from blood samples. Using 11 microsatellite loci, DNA amplification success was 99.1% for eggshell membranes and 97.7% for blood samples. Genetic information within eggshell membrane DNA in comparison to blood DNA was not affected (F(ST) = -0.01735, P = 0.999) by degeneration or possible cross-contamination. Furthermore, neither degeneration nor cross-contamination was apparent in total genotypic comparison of eggshell membrane DNA and blood sample DNA. Our research clearly illustrates that eggshell membranes can be used for population genetic research.

KW - Limosa limosa Population genetics Microsatellite Eggshell membrane DNA quality limosa-limosa-limosa dispersal distances software dynamics Zoology

U2 - 10.1007/s10336-009-0422-2

DO - 10.1007/s10336-009-0422-2

M3 - Article

SN - 0021-8375

VL - 150

SP - 915

EP - 920

JO - Journal of Ornithology

JF - Journal of Ornithology

IS - 4

ER -