USP17- and SCFβTrCP--regulated degradation of DEC1 controls the DNA damage response

Jihoon Kim, Sara D'Annibale, Roberto Magliozzi, Teck Yew Low, Petra Jansen, Indra A Shaltiel, Shabaz Mohammed, Albert J R Heck, Rene H Medema, Daniele Guardavaccaro

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

In response to genotoxic stress, DNA damage checkpoints maintain the integrity of the genome by delaying cell cycle progression to allow for DNA repair. Here we show that the degradation of the basic helix-loop-helix (bHLH) transcription factor DEC1, a critical regulator of cell fate and circadian rhythms, controls the DNA damage response. During unperturbed cell cycles, DEC1 is a highly unstable protein that is targeted for proteasome-dependent degradation by the SCF(βTrCP) ubiquitin ligase in cooperation with CK1. Upon DNA damage, DEC1 is rapidly induced in an ATM/ATR-dependent manner. DEC1 induction results from protein stabilization via a mechanism that requires the USP17 ubiquitin protease. USP17 binds and deubiquitylates DEC1, markedly extending its half-life. Subsequently, during checkpoint recovery, DEC1 proteolysis is reestablished through βTrCP-dependent ubiquitylation. Expression of a degradation-resistant DEC1 mutant prevents checkpoint recovery by inhibiting the downregulation of p53. These results indicate that the regulated degradation of DEC1 is a key factor controlling the DNA damage response.

Originele taal-2Engels
Pagina's (van-tot)4177-85
Aantal pagina's9
TijdschriftMolecular and Cellular Biology
Volume34
Nummer van het tijdschrift22
DOI's
StatusGepubliceerd - 15 nov 2014

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