USP17- and SCFβTrCP--regulated degradation of DEC1 controls the DNA damage response

TY - JOUR

T1 - USP17- and SCFβTrCP--regulated degradation of DEC1 controls the DNA damage response

AU - Kim, Jihoon

AU - D'Annibale, Sara

AU - Magliozzi, Roberto

AU - Low, Teck Yew

AU - Jansen, Petra

AU - Shaltiel, Indra A

AU - Mohammed, Shabaz

AU - Heck, Albert J R

AU - Medema, Rene H

AU - Guardavaccaro, Daniele

N1 - Copyright © 2014, American Society for Microbiology. All Rights Reserved.

PY - 2014/11/15

Y1 - 2014/11/15

N2 - In response to genotoxic stress, DNA damage checkpoints maintain the integrity of the genome by delaying cell cycle progression to allow for DNA repair. Here we show that the degradation of the basic helix-loop-helix (bHLH) transcription factor DEC1, a critical regulator of cell fate and circadian rhythms, controls the DNA damage response. During unperturbed cell cycles, DEC1 is a highly unstable protein that is targeted for proteasome-dependent degradation by the SCF(βTrCP) ubiquitin ligase in cooperation with CK1. Upon DNA damage, DEC1 is rapidly induced in an ATM/ATR-dependent manner. DEC1 induction results from protein stabilization via a mechanism that requires the USP17 ubiquitin protease. USP17 binds and deubiquitylates DEC1, markedly extending its half-life. Subsequently, during checkpoint recovery, DEC1 proteolysis is reestablished through βTrCP-dependent ubiquitylation. Expression of a degradation-resistant DEC1 mutant prevents checkpoint recovery by inhibiting the downregulation of p53. These results indicate that the regulated degradation of DEC1 is a key factor controlling the DNA damage response.

AB - In response to genotoxic stress, DNA damage checkpoints maintain the integrity of the genome by delaying cell cycle progression to allow for DNA repair. Here we show that the degradation of the basic helix-loop-helix (bHLH) transcription factor DEC1, a critical regulator of cell fate and circadian rhythms, controls the DNA damage response. During unperturbed cell cycles, DEC1 is a highly unstable protein that is targeted for proteasome-dependent degradation by the SCF(βTrCP) ubiquitin ligase in cooperation with CK1. Upon DNA damage, DEC1 is rapidly induced in an ATM/ATR-dependent manner. DEC1 induction results from protein stabilization via a mechanism that requires the USP17 ubiquitin protease. USP17 binds and deubiquitylates DEC1, markedly extending its half-life. Subsequently, during checkpoint recovery, DEC1 proteolysis is reestablished through βTrCP-dependent ubiquitylation. Expression of a degradation-resistant DEC1 mutant prevents checkpoint recovery by inhibiting the downregulation of p53. These results indicate that the regulated degradation of DEC1 is a key factor controlling the DNA damage response.

U2 - 10.1128/MCB.00530-14

DO - 10.1128/MCB.00530-14

M3 - Article

C2 - 25202122

SN - 0270-7306

VL - 34

SP - 4177

EP - 4185

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

IS - 22

ER -