Viral genome imaging of hepatitis C virus to probe heterogeneous viral infection and responses to antiviral therapies

Vyas Ramanan, Kartik Trehan, Mei-Lyn Ong, Joseph M Luna, Hans-Heinrich Hoffmann, Christine Espiritu, Timothy P Sheahan, Hamsika Chandrasekar, Robert E Schwartz, Kathleen S Christine, Charles M Rice, Alexander van Oudenaarden, Sangeeta N Bhatia

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Hepatitis C virus (HCV) is a positive single-stranded RNA virus of enormous global health importance, with direct-acting antiviral therapies replacing an immunostimulatory interferon-based regimen. The dynamics of HCV positive and negative-strand viral RNAs (vRNAs) under antiviral perturbations have not been studied at the single-cell level, leaving a gap in our understanding of antiviral kinetics and host-virus interactions. Here, we demonstrate quantitative imaging of HCV genomes in multiple infection models, and multiplexing of positive and negative strand vRNAs and host antiviral RNAs. We capture the varying kinetics with which antiviral drugs with different mechanisms of action clear HCV infection, finding the NS5A inhibitor daclatasvir to induce a rapid decline in negative-strand viral RNAs. We also find that the induction of host antiviral genes upon interferon treatment is positively correlated with viral load in single cells. This study adds smFISH to the toolbox available for analyzing the treatment of RNA virus infections.

Originele taal-2Engels
Pagina's (van-tot)236-47
Aantal pagina's12
TijdschriftVirology
Volume494
DOI's
StatusGepubliceerd - jul 2016

Vingerafdruk Duik in de onderzoeksthema's van 'Viral genome imaging of hepatitis C virus to probe heterogeneous viral infection and responses to antiviral therapies'. Samen vormen ze een unieke vingerafdruk.

Citeer dit